石见穿对肝癌H22荷瘤小鼠肿瘤生长的影响

目的:观察石见穿对肝癌H22小鼠肿瘤生长的影响,并探讨其可能的作用机制.方法:肝癌H22荷瘤小鼠随机分为5组:模型组、石见穿提取物高、中、低(40,10,2.5 g·kg-1 ig)3个剂量组和阳性药环磷酰胺(CTX,0.025 g·kg-1,ip)对照组.连续10d.于第11天观测抑瘤率、脾指数、胸腺指数、血清中肿瘤坏死因子-a(TNF-α)和血骨内皮细胞生长因子(VEGF)的含量.结果:与模型组比较,石见穿提取物高、中、低剂量组的抑瘤率分别为23.83％,48.93％ (P ＜0.01)和27.48 (P＜0.05)；石见穿提取物中、低剂量组脾指数和胸腺指数升高,但差异无统计学意义；石见穿提取物各剂量组血清中TNF-α和VEGF含量下降,以中剂量组VEGF含量下降明显,模型组(28.64±7.18)pg·mL-1,中剂量组(21.22 +5.60)pg·mL-1(P＜0.05).结论:石见穿提取物对肝癌H22荷瘤小鼠肿瘤的生长具有明显的抑制作用,其作用机制可能与下调细胞因子VEGF含量有关.

Inhibitory Effect of Extracts from Salvia chinensis Against H22 Tumor in Mice

Objective:To evaluate the inhibitory effect of extracts from Salvia chinensis against H22 tumor in mice,and analyze the mechanism of inhibiting the growth of tumor.Method: H22 tumor-bearing mice were randomly divided into five groups,they were control group,high,middle and low dosages of extracts from S.chinensis,and positive-control group.All the treatment lasted 10 days.We investigated the inhibition rate,spleen index,thymus index,content of tumor necrosis factor α(TNF-α) and vascular endothelial cell growth factor(VEGF) in serum at the 11th day.Result: In contrast to model group,the inhibition rate of the high,middle,and low dosages of S.chinensis extracts were 23.83%,48.93%(P<0.01) and 27.48(P<0.05),respectively.The spleen index and thymus index of middle and low dosages of S.chinensis extracts rose,but there was not statistical significance.The content of TNF-α and VEGF in serum of administered group were decreased,and the middle dosage of S.chinensis was remarkable(P<0.05).Conclusion: S.chinensis benth extracts could obviously inhibit tumor growth of H22 in tumor-bearing mice,and maybe it involved the down regulation of VEGF.