扶正解毒颗粒对染镍大鼠尿微量蛋白及尿酶活性的影响

目的:观察扶正解毒颗粒(FJG)对硫酸镍(NiSO4)暴露肾损伤大鼠的保护作用.方法:50只Wistar大鼠采用NiSO42.5 mg·kg-1·d-1 ip(连续7d,此后间日1次)制备肾损伤模型,随机分为NiSO4组(模型组)、FJG高、中、低剂量组(20,10,5 g·kg-1·d-1)、二巯基丁二酸(DMSA,50 mg·kg-1·d-1)组.另设NS组(空白对照)及FJG对照组(FJG 10 g·kg-1·d-1).各组大鼠ig 1次/d,共4周.分别采用放射免疫法(RIA)、光电比色法及酶法检测尿α1-微球蛋白(α1-MG)、N-乙酰-β-D氨基葡萄糖甘酶(NAG)及尿肌酐(UCr)的变化.结果:NiSO4组尿α1-MG及NAG活性(NAG/ UCr)较NS组显著增高(P＜0.05和P＜0.01),与NiSO4组相比,FJG各组尿α1-MG及NAG活性则明显降低,尤以FJG高、中剂量组为著(P＜0.05和P＜0.01).结论:尿α1-MG及NAG活性增高与早期镍性肾损伤存在关联,中药FJG对镍性肾损伤大鼠具有较好的保护作用.

Effect of Fuzheng Jiedu Granula on Urinary Micro-protein and Urinary Enzyme in Rats Exposed to Nickel

Objective:To study the effect of Fuzheng Jiedu granula(FJG) on the renal injury in rats exposed to nickel sulfate(NiSO4).Method: Fifty Wistar rats were made into nephrotoxicity models by intraperitoneal injection of NiSO4(2.5 mg·kg-1·d-1).They were randomized into 5 groups,model group,high dose of FJG group(20 g·kg-1·d-1),middle dose of FJG group(10 g·kg-1·d-1),low dose of FJG group(5 g·kg-1·d-1),meso-2,3-dimercaptosuccinic acid(DMSA) group(0.05 g·kg-1·d-1).Twenty Wistar rats were divided into NS group and FJG control group(10 g·kg-1·d-1) randomly.The rats in each group were treated with intragastric administration once every day for four weeks.Urinary α1-microglobulin(α1-MG),urinary N-acetyl-β-D-glucosaminidase(NAG) and urinary creatinine were detected by radioimmunity assay(RIA),photoelectric colorimetry assay and enzymic assay,respectively.Result: In NiSO4 group,urinary α1-MG and NAG activity(NAG/ UCr) were increased remarkably(P<0.05,P<0.01).Compared with that of NiSO4 group,the indexes montioned above in FJG groups decreased obviously(P<0.05,P<0.01).Conclusion: Urinary α1-MG and NAG activity enhancement could be correlated with nickel-induced nephrotoxicity.FJG could protect rats from the renal injury induced by nickel.