石菖蒲水提物对PC12细胞增殖及细胞突起的作用

目的研究石菖蒲水提物对PC12细胞增殖及细胞突起的作用,观察药物是否具有神经营养功能或促进细胞分化的功能。方法采用细胞计数试剂盒检测不同浓度的石菖蒲水提物对细胞活力的影响;采用Image J软件统计PC12细胞的梭型细胞率、细胞分化率、细胞突起长度及阳性细胞分化率,以评价石菖蒲水提物对细胞分化的影响。结果石菖蒲水提物在完全培养条件和低血清培养条件下对PC12细胞增殖有显著的促进作用,对细胞突起的长出、分支、延伸有明显的抑制作用。结论石菖蒲水提物具有一定的神经营养功能。

Effects of Aqueous Extract of Acorus tatarinowii on PC12 Cell Proliferation and Processes

Objective To study the effects of aqueous extract of Acorus tatarinowii on PC12 cell proliferation and processes and to evaluate whether the drug has a neurotrophic function or promotes cell differentiation. Methods Cell Counting Kit-8 was used to determine the effect of different concentrations of aqueous extract of Acorus tatarinowii on the viability of PC12 cells. The spindle cell rate, cell differentiation rate, length of cell processes, and the differentiation rate of positive cells were statistically analyzed using Image J software to evaluate the effect of aqueous extract of Acorus tatarinowii on PC12 cell proliferation. Results The aqueous extract of Acorus tatarinowii significantly promoted PC12 cell proliferation in both complete culture environment and low-serum culture environment and significantly inhibited the growth, branching, and extension of cell processes. Conclusion The aqueous extract of Acorus tatarinowii has a certain neurotropic function.