| Ki-ras CODON 12 MUTATION IN HUMAN COLORECTAL CARCINOMAS |
| |
| 引用本文： | 张宏,田竟生,田华,郑少鹏. Ki-ras CODON 12 MUTATION IN HUMAN COLORECTAL CARCINOMAS[J]. 中国癌症研究, 1993, 5(4): 235-239. DOI: 10.1007/BF03023752 |
| |
| 作者姓名： | 张宏 田竟生 田华 郑少鹏 |
| |
| 作者单位： | Beijing Institute far Cancer Research，Beijing 100034，Beijing Institute far Cancer Research，Beijing 100034，Beijing Institute far Cancer Research，Beijing 100034，Beijing Institute far Neuroaciences，Capitol Institute of Medicine，Beijing 100054 |
| |
| 摘 要： | We examined the incidence of point mutation in codon 12 of Ki-ras oncogene in human colorectal carcinomas by polymerase chain reaction in combination with alot-blot hybridization using mutation-specific ollgodeoxynucleotide as probes. Among 72 colorectal carcinomas, point mutations were found in 36 samples, GGT to TGT in 16 cases and to AGT In 21 cases, one sample contain two different mutations. One of five normal mucosa contains the same mutation as in the adjacent carcinoma, suggesting that genetic alterations may also exist in the regions from which such carcinomas arise.
|
Ki-ras codon 12 mutation in human colorectal carcinomas |
| |
| Hong Zhang,Jingsheng Tian,Hua Tian,Shaopeng Zheng. Ki-ras codon 12 mutation in human colorectal carcinomas[J]. Chinese Journal of Cancer Research, 1993, 5(4): 235-239. DOI: 10.1007/BF03023752 |
| |
| Authors: | Hong Zhang Jingsheng Tian Hua Tian Shaopeng Zheng |
| |
| Affiliation: | Zhang Hong Tian Jingsheng Tian Hua Zheng ShaopengBeijing Institute far Cancer Research,Beijing 100034Beijing Institute far Neuroaciences,Capitol Institute of Medicine,Beijing 100054 |
| |
| Abstract: | We examined the incidence of point mutation in codon 12 of Ki-ras oncogene in human colorectal carcinomas by polymerase chain reaction in combination with alot-blot hybridization using mutation-specific ollgodeoxynucleotide as probes. Among 72 colorectal carcinomas, point mutations were found in 36 samples, GGT to TGT in 16 cases and to AGT In 21 cases, one sample contain two different mutations. One of five normal mucosa contains the same mutation as in the adjacent carcinoma, suggesting that genetic alterations may also exist in the regions from which such carcinomas arise. |
| |
| Keywords: | PCR Slot-blot hybridization Ki-ras gene Point mutation. |
| 本文献已被 CNKI SpringerLink 等数据库收录！ |
