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利多卡因对短暂脑缺血后海马区细胞凋亡的影响
引用本文:宋瑞平,吴新民. 利多卡因对短暂脑缺血后海马区细胞凋亡的影响[J]. 中华麻醉学杂志, 2000, 20(9): 546-548
作者姓名:宋瑞平  吴新民
作者单位:100034,北京大学第一医院麻醉科
摘    要:目的 探讨利多卡因对短暂脑缺血后海马区迟发性神经元降解的影响。方法 脑缺血模型为兔脑四血管夹闭模型,25只家兔随机分成三组:假手术组(Sh,n=5);缺血组(Is,n=10),夹闭双侧颈总动脉和椎动脉5min后恢复脑灌注;利多卡因组(Li,n=10),夹闭颈总和椎动脉前5min给予利多卡因10mg/kg。3组均于3d后取脑行病理HE染色和TUNEL染色,对民区阳性细胞进行计数。结果 HE染色发生缺

关 键 词:麻醉药 利多卡因 短暂性脑缺血 细胞凋亡
修稿时间:2000-03-14

Effect of lidocaine on hippocampus neural apoptosis after transient global cerebral ischemia
SONG Ruiping,WU Xinmin. Effect of lidocaine on hippocampus neural apoptosis after transient global cerebral ischemia[J]. Chinese Journal of Anesthesilolgy, 2000, 20(9): 546-548
Authors:SONG Ruiping  WU Xinmin
Affiliation:SONG Ruiping,WU Xinmin(Department of Anesthesiology, First Affiliated Hospital of Bojing University,Beijing 100034)
Abstract:Objective To evaluate the effect of lidocaine on delayed neuroal death after global cerebral ischemia. Methods Global cerebral ischemia was induced by the occlusions of bilateral commom carotid and vertebral arteries . Twenty-five rabbits were randomly divided into three groups:sham operative group (SH group,n=5) without the four-vessel occlusion; ischemia group(IS group,n=10) with intravenous normal saline of 15 ml followed by the four-vessel occlusion for 5 min; lidocaine group (LI group,n=10) with intravenous lidocaine of 10mg/kg followed by the four-vessel occlusion for 5 min. Three days later, every cerebrum was resected and stained using terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling (TUNEL) and hematoxylin-eosin (HE) methods,to count the amount of the positive stained cells of hippocampus. Results The positive cell amount of HE staining (ischemic change cells) was correlated with that of TUNEL staining (apoptosis cells) (r=0.915).Compared with that in SH group, the amount of apoptosis cells increased significantly in IS and LI groups (P<005), which was obviously higher in LI group than that in IS group (P<0.05). Conclusions Lidocaine can aggravate hippocampus neural apoptosis after transient global cerebral ischemia.
Keywords:Apoptosis   Cerebral ischemia, transient   Lidocaine
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