中药醒鼻凝胶滴剂治疗变应性鼻炎的药效学研究

目的:观察中药醒鼻凝胶滴剂对豚鼠变应性鼻炎(AR)的干预作用及与白三烯E4(LTE4)、免疫球蛋白E(IgE)的关系,探讨醒鼻凝胶滴剂干预治疗的可能机理,并为该药的临床应用提供药效学实验依据。方法:以卵清蛋白(OVA)+氢氧化铝基础致敏、局部以2%OVA激发复制豚鼠AR模型,实验豚鼠分为正常对照组(A组)、模型组(B组)和治疗组(C、D、E组),每组13只,C、D、E组分别予醒鼻凝胶滴剂Ⅰ号、醒鼻凝胶滴剂Ⅱ号及雷诺考特滴鼻给药治疗,A组及B组予生理盐水。每日3次,连续11天。比较各组豚鼠AR的行为学表现,测定血清中LTE4、IgE含量,观察鼻黏膜组织病理形态学和免疫学改变。结果:C、D、E组豚鼠的AR行为学评分明显减低(与B组比较P<0.01),血清中LTE4、IgE含量降低(与B组比较P<0.01),鼻黏膜嗜酸性粒细胞计数显著降低(与B组比较P<0.01),核因子-κB(NF-κB)免疫组化结果各组间比较无显著差异。结论:中药醒鼻凝胶滴剂治疗AR豚鼠有显著疗效,其作用机制是通过降低机体的LTE4、IgE含量及鼻黏膜的嗜酸性粒细胞等因素产生。

Pharmaco-dynamics research of Chinese medicine Xingbi Gelation Drop in treating allergic rhinitis

Objective:To observe the intervention effect of traditional Chinese medicine Xingbi Gelation Drop on guinea pig with allergic rhinitis(AR),and its relation with Leukotriene E4 and Immunoglobulin E,to investigate the possible mechanism of Xingbi Gelation Drop,meanwhile,to provide pharmaco-dynamics experimental basis for the clinical application of the medicine. Methods:The guinea pig AR model was established by sensitizing guinea pigs with ovalbumin (OVA) and aluminum hydroxide,and exciting with OVA in two p...