WHBE兔与日本大耳白兔冠脉结扎致心肌缺血模型的比较研究

目的 复制WHBE兔冠脉结扎所致心肌缺血模型,并与日本大耳白兔比较其心血管系统的敏感性.方法 雄性WHBE兔和日本大耳白兔各20只,随机分成伪手术组和模型组,每组10只,伪手术组只开胸不结扎,模型组采用冠脉结扎复制心肌缺血模型,各组分别在造模前,造模后60 min观察sT段抬高程度,并取血测定血浆AST、CK、LDH活性的变化;用NBT染色法,结合图像分析软件计算心肌缺血梗死程度.结果 与伪手术组比,冠脉结扎后WHBE兔和日本大耳白兔模型组ST段明显抬高（P〈0.01）,梗死面积程度明显增加（P〈0.01）,且血浆AST、CK、LDH活性水平均显著升高（P〈0.05,P〈0.01）;与日本大耳白兔比,WHBE兔冠脉结扎所致心肌觖血的ST段、梗死面积程度和血浆AST、CK、LDH活性水平的上升变化程度均略高于日本大耳白兔,以梗死面积程度变化最为显著（P〈0.05）.结论 WHBE兔对冠脉结扎所致心肌缺血较为敏感,是比较理想的心肌缺血模型,更适合于心血管缺血性疾病的研究.

Comparative Study on Myocardial Ischemia Model Induced by Ligation of Coronary Artery in WHBE Rabbits and Japanese White Rabbits

Objective To establish a myocardial ischemia model induced by ligation of coronary artery in WHBE rabbits,, and to investigate its cardiovascular system sensitivity through comparing to Japanese white rabbits. Methods Male WHBE rabbits and Japanese white rabbits were randomly divided into sham operation group and model group （n=10 in each）. The sham operation group was only chest opened but not ligated and the myocardial ischemia model was induced by ligation of coronary artery. The degree of ST-segment elevation and activity changes of blood plasma AST, CK, LDH were observed before, at, and after making model for 60 min in each group, and myocardial infarction size was measured by NBT staining and image analysis software. Results Compared with sham operation group, the ST-segment was significantly elevated （P〈0.01）, and infarction size was also increased （P〈0.01）, the blood plasma AST, CK, LDH activities were markedly raised after ligation of coronary artery in WHBE rabbits and Japanese white rabbits model group （P〈0.05, P〈0.01）. Compared with Japanese white rabbit, the ST-segment, infarction size and blood plasma AST, CK, LDH activities in WHBE rabbits induced by ligation of coronary artery were slightly higher than those of Japanese white rabbits, especially for infarction size （P〈0.05）. Conclusion WHBE rabbit is more sensitive for myocardial ischemia induced by ligated coronary artery and ideal myocardial infarction model, and adapted to research of cardiovascular disease.