| HSP60和HSP70在严重烧伤大鼠胃黏膜的表达及对胃黏膜的保护作用 |
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| 引用本文： | 张红艳,吕农华,谢勇,郭光华,詹剑华,陈江.HSP60和HSP70在严重烧伤大鼠胃黏膜的表达及对胃黏膜的保护作用[J].江西医学院学报,2008,48(2):34-38. |
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| 作者姓名： | 张红艳 吕农华 谢勇 郭光华 詹剑华 陈江 |
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| 作者单位： | 南昌大学第一附属医院烧伤科;南昌大学第一附属医院消化科; |
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| 摘 要： | 目的观察严重烧伤大鼠胃黏膜热休克蛋白60和70(HSP60、HSP70)的表达变化及热休克预处理(HS)对严重烧伤大鼠胃黏膜的影响,探讨热休克预处理诱导HSP60、HSP70过量表达后对严重烧伤大鼠胃黏膜的保护作用。方法将Wistar大鼠随机分为2大组:①烧伤组(B组)40只大鼠烧伤后即制作急性胃黏膜损伤模型;另取8只大鼠不烧伤,作为正常对照组(伤前);②HS+烧伤组(HB组)40只大鼠于烧伤前20 h行HS,另取8只大鼠只行HS不烧伤,作为实验对照组(伤前)。各组于伤后3、6、12、24、48 h处死大鼠(B组、HB组每时相点8只大鼠),留取标本检测胃黏膜损伤指数(UI)、HSP60、HSP70的变化,并进行相关分析。结果大鼠严重烧伤后胃黏膜HSP60、HSP70表达迅速升高,随后开始下降,伤后24 h降至最低,此时胃黏膜损伤程度最严重(UI=12.74±1.92)。HB组大鼠较B组大鼠HSP60、HSP70表达均显著增强,胃黏膜损伤程度则明显减轻(P<0.05或P<0.01)。UI与HSP70呈显著负相关(r=-0.794,P<0.05),UI与HSP60无相关性(r=-0.625,P>0.05)。结论热...
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| 关 键 词： | 烧伤 应激性溃疡 热休克蛋白 胃黏膜 动物 实验 大鼠 |
| 文章编号： | 1000-2294(2008)02-0034-03 |
| 修稿时间： | 2007年12月12 |
The Expressions of Heat Shock Proteins 60 and 70 and Their Protective Effect on the Gastric Mucosa of Burned Rats |
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| ZHANG Hong-yana,LV Nong-huab,XIE Yongb,GUO Guang-huaa,Zhan Jian-huaa,CHEN Jiangb.The Expressions of Heat Shock Proteins 60 and 70 and Their Protective Effect on the Gastric Mucosa of Burned Rats[J].Acta Academiae Medicinae Jiangxi,2008,48(2):34-38. |
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| Authors: | ZHANG Hong-yana LV Nong-huab XIE Yongb GUO Guang-huaa Zhan Jian-huaa CHEN Jiangb |
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| Institution: | ZHANG Hong-yana,LV Nong-huab,XIE Yongb,GUO Guang-huaa,Zhan Jian-huaa,CHEN Jiangb(a.Department of Burns,b.Department of Gastroenterology,the First Affiliated Hospital of Nanchang University,Nanchang 330006,China) |
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| Abstract: | Objective To observe the changes of the expressions of heat shock proteins(HSP) 60 and 70 in the gastric mucosa of burned rats and the effect of heat shock preconditioning(HS) on the gastric mucosa of burned rats in order to explore the protection of HSP 60 and 70.MethodsNinety-six wistar rats were divided randomly into two groups:(1)burn group(n=40,burned and then being made into the model of acute gastric mucosal lesion)and another 8 normal rats without burn were employed as blank control(preburn);(2)HB g... |
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| Keywords: | burn acute gastric mucosa lesion heat shock protein gastric mucosa animal laboratory rats |
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