六味地黄丸含药血清对树突状细胞增殖的影响

目的：研究六味地黄丸含药血清对SD大鼠骨髓源性树突状细胞（bone marrow-derived dendritic cells，BMDC）增殖的影响。方法：制备六味地黄丸含药血清，观察含药血清作用下的细胞生长情况，进行细胞计数及细胞活力测定；用四甲基偶氮唑盐（MTT）法，检测不同浓度、不同时间六味地黄丸含药血清作用下的细胞增殖变化，寻找最佳六味地黄丸浓度和作用时间；ELISA法检测细胞上清中树突状细胞分泌细胞因子IL-12的浓度；混合淋巴细胞反应（MLR）检测其刺激T淋巴细胞活化和增殖能力。结果：与空白对照组比较，成熟型DC含药血清组显著促进DC细胞增殖，且这种增殖在含药浓度15%，作用时间48 h时最为显著。其IL-12分泌量显著提高（P<0.05）。六味地黄丸含药血清培养的成熟型DC对异基因T淋巴细胞具有增殖刺激作用，且随着细胞数量的增加作用增强（P<0.05），呈量效关系。结论：六味地黄丸含药血清具有诱导大鼠骨髓源性树突状细胞增殖的作用。

Effects of Liuwei Dihuang Wan Containing Serum on Dendritic Cell

Objective: To study Liuwei Dihuang wan drug serum on SD rat bone marrow-derived dendritic cells (BMDC) proliferation. Method: Made Liuwei Dihuang wan drug serum, drug serum was observed under the action of cell growth, cell count and cell viability test.Using tetrazolium salt (MTT) method to detect different concentrations and time Liuwei Dihuang wan pills drug serum changes under the action of cell proliferation, finding the best Liuwei Dihuang wan concentration and time. ELISA assay supernatants of dendritic cells secrete cytokines IL-12 concentration. mixed lymphocyte reaction (MLR) test which stimulate T cell activation and proliferation. Result: Compared with the control group, mature DC-containing serum significantly promote the DC cell proliferation, and this proliferation of drug-containing concentrations of 15%, reaction time 48h of the most significant. Its IL-12 secretion significantly increased (P<0.05). Liuwei Dihuang wan drug serum mature DC cultured allogeneic lymphocytes proliferation stimulating effect, and with the enhanced role of the increase in the number of cells (P<0.05), showed a dose-effect relationship. Conclusion: Liuwei Dihuang wan drug serum has induced the role of rat bone marrow dendritic cells proliferation.