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| 乳腺癌MCF-7细胞系中侧群细胞分选及其生物学特性 | |
| 引用本文： | 孙鑫,李平,张梅,陈娇. 乳腺癌MCF-7细胞系中侧群细胞分选及其生物学特性[J]. 中国基层医药, 2012, 19(13): 1927-1928 |
| 作者姓名： | 孙鑫 李平 张梅 陈娇 |
| 作者单位： | 1. 230032 安徽省合肥,安徽医科大学;安傲医科大学附属省立医院中西医结合肿瘤科 2. 安徽医科大学附属省立医院中西医结合肿瘤科 3. 安徽医科大学附属省立医院中西医结合肿瘤科;安徽中医学院 |
| 基金项目： | 国家中医药管理局中医肿瘤病学重点学科经费资助 |
| 摘 要： | 目的 分离乳腺癌MCF-7细胞系中的侧群细胞(SP)并观察其生物学特性.方法 利用流式细胞荧光分选法将乳腺癌MCF-7细胞系分成SP和非SP细胞两个亚群.对两个亚群细胞采用软琼脂克隆形成实验观察其增殖能力.结果 MCF-7细胞株中分选出SP细胞占(6.5±0.4)％;SP细胞的体外克隆形成率为(38.5±9.4)％,高于非SP细胞的(8.4±2,6)％(t=5.34,P＜0.05).结论 乳腺癌MCF-7细胞中的SP细胞富集了乳腺癌于细胞,其增殖能力强于非SP细胞,表明SP表型的肿瘤细胞在乳腺癌的生长中具有重要的地位. |
| 关 键 词： | MCF-7乳腺癌细胞系 侧群细胞 增殖 |
Sorting of side population cells from breast cancer MCF-7 cell line and its biological characteristics |
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| SUN Xin , LI Ping , ZHANG Mei , CHEN Jiao. Sorting of side population cells from breast cancer MCF-7 cell line and its biological characteristics[J]. Chinese Journal of Primary Medicine and Pharmacy, 2012, 19(13): 1927-1928 | |
| Authors: | SUN Xin LI Ping ZHANG Mei CHEN Jiao |
| Affiliation: | . 2009 Geriatrics Craduate of Anhui Medical University, Hefei, Anhui 230032, China |
| Abstract: | Objective To separate the side population cells （SP） from breast cancer MCF-7 cell line, and observe its biological characteristics. Methods Flow cytometry and Hoechst 33342 dye efflux assay were used to iso- late SP ceils and non-SP ceils from the MCF-7 cell line of human breast cancer. Tumorigenicity of the two snbpopula- tions was observed by a soft agar cloning method. Results The results of FACS analysis indicated that （6.5± 0.4 ） % of the MCF-7 cells were SP cells ; The vitro colony formation rate of SP cells was （ 38.5 ± 9.4） %, and higher than that of non-SP cells （ 8.4± 2.6） % （ t = 5.34, P 〈 0.05 ）. Conclusion The SP ceils sorted from MCF-7 cell line enriched tumor stem cells, which exhibited high tumorigenicity. It indicated that SP cells should play a principal role in breast cancer. |
| Keywords: | MCF-7 breast cancer stem cell Side population cell Proliferation |
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