Retardation in somatosensory cortex development induced by postnatal BrdU treatment in mice |
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| Authors: | Melinda Bé ldi,Jó zsef Taká cs,Gyö rgy Bá rdos,Ildikó Vilá gi |
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| Affiliation: | 1. Department of Physiology and Neurobiology, Eötvös Loránd University, Pázmány Péter sétány 1/C, H-1117 Budapest, Hungary;2. Neurobiology Research Group of the Hungarian Academy of Sciences at the Semmelweis University, T?zoltó Street 58, H-1094 Budapest, Hungary |
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| Abstract: | Cerebral dysgeneses are in the background of several neurological and mental disturbances. The aim of the present study was to investigate structural and activity changes following disturbed postnatal neuronal development in mice. Newborn C57Bl6 mice were exposed to 5-bromo-2′-deoxyuridine (BrdU: daily 50 μg/g body weight) during a period between postnatal days P0–P5 or P0–P11, respectively, and neuronal malformation and malfunctioning of somatosensory (barrel field) cortex was analyzed in adolescent animals. Alterations in histological architecture of interneuronal and glial elements were studied and correlated with electrophysiological modifications. Between P30 and P35 days litters underwent ex vivo electrophysiological experiments to examine the changes in basic excitability and in synaptic efficacy. Parallel immunohistochemistry was performed to detect BrdU, GABA and GFAP. |
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| Keywords: | ACSF, artificial cerebrospinal fluid BrdU, 5-bromo-2&prime -deoxyuridine CB, calbindin D-28k DAB, 3,3&prime -diamino-benzidine GABA, γ-aminobutyric acid GFAP, glial fibrillary acidic protein I&ndash O curve, stimulus intensity-evoked response (input&ndash output) curve MFR, Mg2+-free Ringer solution PBS, phosphate-buffered saline PMBSF, postero-medial barrel subfield S1BF, primary somatosensory barrel field cortex T, stimulus threshold of evoked response wm, white matter |
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