细胞外基质及其筑构对平滑肌细胞增殖的影响

目的:研究细胞外基质层粘连蛋白(Laminin,LN),纤维连接蛋白(Fibronecfin,FN)和三维基膜基质Matrigel胶对平滑肌细胞增殖的作用。方法:将用酶消化法分离的兔主动脉平滑肌细胞种植在LN和FN上及三维基膜基质Matrigel胶里,然后用Brdu法检测各平滑肌细胞的增殖率。结果:生长在FN上的平滑肌细胞增殖率最高(24%),生长在LN上的平滑肌细胞增殖率次之(17%),而生长在三维基膜基质胶里的平滑肌细胞未出现细胞增殖,另外LN较FN有明显的促进平滑肌细胞分化成熟的作用。结论:细胞外基质成分和基质筑构对平滑肌细胞的增殖与分化具有重要的调节作用。

The effect of extracellular matrix and its oomponents on the proliferation of wascular smooth muscle cell

Objective:To study the effect of LN, FN and Matrigel on proliferation of vascular smooth muscle cells. Methods: Brdu technique was used to detect the proliferation rate of smooth muscle cells obtained from aorta of rabbits cultured on FN, LN and Matrigel. Results:The proliferation rate of smooth muscle cells growing on FN was about 24%, 1.4 fold higher than that on LN (17%). No proliferation was detected for smooth muscle cell growing inside Matrigel. In addition, the effect of LN on promoting differentiation and maturation of smooth muscle cells was observed. Conclusions:our data demonstrates that the components of extracellular matrix, as well their three dimensional structures are important for proliferation of vascular smooth muscle cells.