老年肺癌患者肿瘤细胞和外周血淋巴细胞体外化疗药敏试验的相关性

目的 探讨老年非小细胞肺癌患者体外肿瘤细胞和外周血淋巴细胞对化疗药物敏感性的关系.方法 选取52例老年非小细胞肺癌患者,采用MTT法体外药敏试验,检测外周血淋巴细胞和肿瘤细胞对13种化疗药物的敏感性.结果 老年非小细胞肺癌患者外周血淋巴细胞对顺铂(CDDP)、卡铂(CBP)、奥沙利铂(LO HP)、紫杉醇(PTX)、环磷酰胺(CTX)、异环磷酰胺(ICTX)、吡喃阿霉素(THP)、依托泊苷(VP-16)、吉西他滨(GEM)、长春新碱(VCR)、长春瑞滨(NVB)等11种化疗药物的敏感性与非小细胞肺癌肿瘤细胞的药敏结果有相关性.而对阿霉素、羟基喜树碱这2种化疗药物的敏感结果无相关性.结论 老年非小细胞肺癌患者可以应用外周血淋巴细胞代替肿瘤细胞进行体外化疗药物敏感试验.

Study on chemosensitivity test in vitro between peripheral blood lymphocytes and tumor cells in elderly lung cancer

Objective To explore the correlation of chemosensitivity in vitro of anti-cancer drugs between peripheral blood lymphocytes and tumor cells＇in non-small cell lung cancer of elderly patients. Methods The MTT method was used to test the sensitivity of the peripheral blood lymphocyte and the tumor cells of 52 patients with non- small cell lung cancer to 13 kinds of anti-cancer drugs. Results The sensitivity test in eleven drugs include CDDP, CBP, LOHP, PTX, CTX, ICTX, THP, VP-1 6, GEM, VCR and NVB in the peripheral blood lymphocyte were associated with that in the tumor ceils. But no dependablity in two drugs, ADM and HCPT. Conclusion The peripheral blood lymphocytes could be replace tumor cells to test the chemosensitivity of anti-cancer drugs in patients with non-small cell lung cancer.