几种因素对大鼠再生肝摄取氨基酸能力的影响——离体肝灌流的实验研究

本实验选用大鼠肝部分切除后24小时的残余肝离体灌流,研究了四种因素对残余肝摄取氨基酸能力的影响。结果表明:1.肝部分切除后24小时,残余肝摄取氨基酸的量增加。肝脏摄取氨基酸能力的增强依赖于肝细胞蛋白质合成的增加。在一定范围内,肝脏摄取氨基酸的量随肝切除量的增加而增多。2.胰岛素能促进残余肝摄取氨基酸,高血糖素无此作用,但高血糖素与胰岛素联合施用则使残余肝摄取氨基酸的能力进一步增强。3.大鼠再生肝提取液中含有某种能促进残余肝摄取氨基酸的物质。

Effects of several factors on ability of amino acid uptake by regenerating rat liver

24hr after partial hepatectomy, the isolated remnant liver of rat was perfused to study the effects of several factors on the ability of amino acid intake. The results were as follows: (1). Twenty-four hours after operation the hepatic uptakes of amino acid increased in the hepatectomized groups. The ability of enhanced amino acid uptake by remnant liver depended on hepatic protein synthesis. In a certain range of hepatectomy there was a positive correlation between the amounts of liver which had been cut off and the amounts of amino acid uptake by remnant liver. (2). The addition of insulin alone to the perfusate could stimulate amino acid uptakes by remnant liver and the addition of glucagon alone had no effect. But, when insulin was added to the perfusate in combination with glucagon the hepatic uptake of amino acid was much higher. (3). The extract from regenerating rat liver rather than that from normal adult rat liver may contain certain factor(s) which can stimulate amino acid uptakes by remnant liver.