| Effect of type-2 astrocytes on the viability of dorsal root ganglion neurons and length of neuronal processes简 |
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| 引用本文： | Chunling Fan,Hui Wang,Dan Chen,Xiaoxin Cheng,Kun Xiong,Xuegang Luo,Qilin Cao. Effect of type-2 astrocytes on the viability of dorsal root ganglion neurons and length of neuronal processes简[J]. 中国神经再生研究, 2014, 9(2): 119-128. DOI: 10.4103/1673-5374.125339 |
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| 作者姓名： | Chunling Fan Hui Wang Dan Chen Xiaoxin Cheng Kun Xiong Xuegang Luo Qilin Cao |
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| 基金项目： | This study was supported by the NIH Foundation of the USA, No. RO1 NS061975, and the Natural Science Foundation of Hunan Province in China, No. 11JJ6077. |
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| 摘 要： | The role of type-2 astrocytes in the repair of central nervous system injury remains poorly un- derstood. In this study, using a relatively simple culture condition in vitro, type-2 astrocytes, differentiated from oligodendrocyte precursor cells by induction with bone morphogenetic pro- tein-4, were co-cultured with dorsal root ganglion neurons. We examined the effects of type-2 astrocytes differentiated from oligodendrocyte precursor cells on the survival and growth of dorsal root ganglion neurons. Results demonstrated that the number of dorsal root ganglion neurons was higher following co-culture of oligodendrocyte precursor cells and type-2 astrocytes than when cultured alone, but lower than that of neurons co-cultured with type-1 astrocytes. The length of the longest process and the length of all processes of a single neuron were shortest in neurons cultured alone, followed by neurons co-cultured with type-2 astroc~es, then neurons co-cultured with oligodendrocyte precursor cells, and longest in neurons co-cultured with type-1 astrocytes. These results indicate that co-culture with type-2 astrocytes can increase neuronal survival rate and process length. However, compared with type-1 astrocytes and oligodendrocyte precursor cells, the promotion effects of type-2 astrocytes on the growth of dorsal root ganglion neurons were weaker.
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| 关 键 词： | 背根神经节神经元 星形胶质细胞 存活率 2型 长度 中枢神经系统损伤 前体细胞 培养条件 |
Effect of type-2 astrocytes on the viability of dorsal root ganglion neurons and length of neuronal processes |
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| Chunling Fan,Hui Wang,Dan Chen,Xiaoxin Cheng,Kun Xiong,Xuegang Luo,Qilin Cao. Effect of type-2 astrocytes on the viability of dorsal root ganglion neurons and length of neuronal processes[J]. Neural Regeneration Research, 2014, 9(2): 119-128. DOI: 10.4103/1673-5374.125339 |
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| Authors: | Chunling Fan Hui Wang Dan Chen Xiaoxin Cheng Kun Xiong Xuegang Luo Qilin Cao |
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| Affiliation: | [1]Department of Human Anatomy and Neurobiology, Xiangya School of Medicine, Central South University, Changsha, Hunan Province, China [2]The Vivian L Smith Department of Neurosurgery, UT Medical School at Houston, Houston, TX, USA |
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| Abstract: | The role of type-2 astrocytes in the repair of central nervous system injury remains poorly understood. In this study, using a relatively simple culture condition in vitro, type-2 astrocytes, differentiated from oligodendrocyte precursor cells by induction with bone morphogenetic protein-4, were co-cultured with dorsal root ganglion neurons. We examined the effects of type-2 astrocytes differentiated from oligodendrocyte precursor cells on the survival and growth of dorsal root ganglion neurons. Results demonstrated that the number of dorsal root ganglion neurons was higher following co-culture of oligodendrocyte precursor cells and type-2 astrocytes than when cultured alone, but lower than that of neurons co-cultured with type-1 astrocytes. The length of the longest process and the length of all processes of a single neuron were shortest in neurons cultured alone, followed by neurons co-cultured with type-2 astrocytes, then neurons co-cultured with oligodendrocyte precursor cells, and longest in neurons co-cultured with type-1 astrocytes. These results indicate that co-culture with type-2 astrocytes can increase neuronal survival rate and process length. However, compared with type-1 astrocytes and oligodendrocyte precursor cells, the promotion effects of type-2 astrocytes on the growth of dorsal root ganglion neurons were weaker. |
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| Keywords: | nerve regeneration spinal cord injury oligodendrocyte oligodendrocyte precursor cells astrocytes bone morphogenetic protein neurons neurites dorsal root ganglion NIH grant neuralregeneration |
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