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| 用荧光偏振免疫法和微生物法比较研究庆大霉素在人体内的药代动力学 | |
| 引用本文： | 蔡卫民,陈刚. 用荧光偏振免疫法和微生物法比较研究庆大霉素在人体内的药代动力学[J]. 药学学报, 1990, 25(2): 81-84 |
| 作者姓名： | 蔡卫民 陈刚 |
| 作者单位： | 南京军区南京总医院药理科，南京军区南京总医院药理科 南京 210002，南京 210002 |
| 摘 要： | 本文用新建立的荧光偏振免疫法(FPIA)和微生物法(MA)平行测定90例临床庆大霉素血清浓度,线性回归分析发现两种方法有较高的相关性(r=0.98)。但用MA法测得庆大霉素的平均浓度明显高于FPIA的平均浓度。5例病人庆大霉素药动学研究表明,两种方法所得血浓数据经计算机拟合符合单室模型,动力学参数Vd,k,T_(1/2)和CL及由此推算的给药剂量均无显著差异(p>0.05)。 |
| 关 键 词： | 庆大霉素 荧光偏振免疫法 微生物法 药代动力学 |
| 收稿时间： | 1989-02-10 |
USE OF FLUORESCENCE POLARIZATION IMMUNOASSAY AND MICROBIOLOGICAL ASSAY IN THE STUDY OF GENTAMICIN PHARMACOKINETICS IN MAN AND A COMPARISON OF THE RESULTS |
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| WM Cai and G Chen. USE OF FLUORESCENCE POLARIZATION IMMUNOASSAY AND MICROBIOLOGICAL ASSAY IN THE STUDY OF GENTAMICIN PHARMACOKINETICS IN MAN AND A COMPARISON OF THE RESULTS[J]. Acta pharmaceutica Sinica, 1990, 25(2): 81-84 | |
| Authors: | WM Cai and G Chen |
| Affiliation: | General Hospital of Nanjing Armed Forces. |
| Abstract: | Ninety serum samples of gentamicin were determined by fluorescence polarization immunoassay (FPIA) and microbiological assay (MA) and the results were compared. Liner regression analysis showed that the two methods were highly correlated with a correlation coefficient of 0.98. The mean concentration of gentamicin as determined by MA method was significantly higher than that by FPIA method. Serum concentration-time data derived from 5 patients were fitted to a one-compartment model. No significant differences in the pharniacokinetic parameters and estimated dosages were observed. The FPIA method was shoran to be as correct and reliable as the MA method in estimating gentamicin pharmacokinetic parameters. |
| Keywords: | Gentamicin Fluorescence polarization immunoassay Microbiological assay Pharmacokinetics |
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