| 抗纤灵方对大鼠单侧输尿管梗阻所致肾纤维化的防治作用 |
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| 引用本文： | 刘克剑,张悦,李靖,何立群,庞惠芳,徐伟珍. 抗纤灵方对大鼠单侧输尿管梗阻所致肾纤维化的防治作用[J]. 上海中医药大学学报, 2009, 23(5): 44-47 |
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| 作者姓名： | 刘克剑 张悦 李靖 何立群 庞惠芳 徐伟珍 |
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| 作者单位： | 上海中医药大学科技实验中心,上海,201203;上海中医药大学科技实验中心,上海,201203;上海市中医药研究院肾病研究所,上海,201203;上海市中医药研究院肾病研究所,上海,201203 |
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| 基金项目： | 国家自然科学基金资助项目(30371834);;上海市高等学校科学技术发展基金资助项目(02CK10);;上海市教委重点学科建设资助项目(J50301) |
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| 摘 要： | 目的：探讨中药抗纤灵方对大鼠单侧输尿管结扎（UUO）所致肾纤维化的防治作用及机制。方法：用单侧输尿管结扎术建立大鼠肾纤维化模型,动态观察4周。治疗组以抗纤灵方在造模的同时开始灌胃,1次／d,共4周。治疗的第2周末检测BUN、Scr含量等指标,观察肾功能变化;第4周末采用HE染色、丽春红染色观察。肾组织病理变化和胶原纤维沉积变化;采用RT-PCR法观察梗阻肾皮质C-Met、HGF、Erk1 mRNA表达。结果：模型组大鼠BUN、Scr明显升高;肾小管和间质的结构基本消失,呈现弥漫的细胞增殖;肾间质胶原纤维增生和大量炎细胞浸润;肾组织C-Met mRNA表达显著上调,而HGF mRNA水平显著下调。中药抗纤灵方治疗组肾脏炎性细胞浸润及间质损伤均轻于模型组;BUN含量显著降低,Scr含量趋向正常;肾组织C-Met mRNA表达明显减少,HGF mRNA水平明显上调。ERK总基因水平的表达在假手术组、模型组及中药治疗组之间比较无明显差异。结论：抗纤灵方可能与抑制肾组织C-Met mRNA表达并增加抗肾纤维化的细胞因子HGF mRNA表达来改善大鼠梗阻性肾间质纤维化及肾功能有关。
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| 关 键 词： | 抗纤灵方 单侧输尿管梗阻 肾间质纤维化 大鼠 肝细胞生长因子 |
Effects of Kangxian Ling Decoction on Renal Interstitial Fibrosis Induced by Unilateral Ureteral Obstruction in Rats |
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| LIU Ke-jian ZHANG Yue, LI Jing HE Li-qun PANG Hui-fang XU Wei-zhen. Effects of Kangxian Ling Decoction on Renal Interstitial Fibrosis Induced by Unilateral Ureteral Obstruction in Rats[J]. Acta Universitatis Traditionis Medicalis Sinensis Pharmacologiaeque Shanghai, 2009, 23(5): 44-47 |
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| Authors: | LIU Ke-jian ZHANG Yue LI Jing HE Li-qun PANG Hui-fang XU Wei-zhen |
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| Affiliation: | LIU Ke-jian1 ZHANG Yue1,2 LI Jing1 HE Li-qun2 PANG Hui-fang1 XU Wei-zhen11.Shanghai University of Traditional Chinese Medicine2.Institute of Kidney Disease,Shanghai Academy of Traditional Chinese Medicine |
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| Abstract: | Objective:To study the effects of Kangxian Ling Decoction(KLD) on renal interstitial fibrosis induced by unilateral ureteral obstruction(UUO) in rats.Methods: The renal interstitial fibrosis model was established in rats by UUO and was observed dynamically for 4 weeks.The rats in treatment group were treated with KLD from the establishing of model once a day for 4 weeks.The level of serum creatinine(Scr) and serum urea nitrogen(BUN) were detected to evaluate the kidney function at the end of the 2nd week.... |
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| Keywords: | ＂Kangxian Ling Decoction＂ （KLD） unilateral ureteral obstruction（UUO） renal interstitial fibrosis rat hepatocyte growth factor（HGF） |
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