| 150例男性不育患者Y染色体AZF基因微缺失分析 |
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| 引用本文： | 莫毅,梁方方,檀大羡,吕福通,陈美佳,何科峰,谢丹尼. 150例男性不育患者Y染色体AZF基因微缺失分析[J]. 中国计划生育学杂志, 2010, 18(9): 541-543. DOI: 10.3969/j.issn.1004-8189.2010. |
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| 作者姓名： | 莫毅 梁方方 檀大羡 吕福通 陈美佳 何科峰 谢丹尼 |
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| 作者单位： | 1. 广西壮族自治区人口和计划生育研究中心,南宁,530021
2. 广西壮族自治区畜牧研究所 |
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| 基金项目： | 国家"十一五"科技基础工作专项 |
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| 摘 要： | 目的:探讨Y染色体无精子因子(AZF)微缺失检测在男科诊断中的应用价值。方法:采用多重聚合酶链反应技术及琼脂糖凝胶电泳方法,对150例男性不育患者AZF基因的3个STS位点进行分析。结果:150例中22例(14.67%)AZF基因位点发生微缺失,其中AZFc位点缺失最多,其次为AZFb,AZFa最低。结论:AZF基因微缺失与精子缺陷发生密切相关,对男性不育患者进行Y染色体AZF区域微缺失检测是必要的。
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| 关 键 词： | Y染色体微缺失 无精子因子 男性不育 |
Analysis of azoospermia factor microdeletions on Y chromosome in 150 infertility patients |
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| Affiliation: | Mo Yi,Liang Fangfang,Tan Daxian,et al.Research center of population and family planning of Guangxi Zhuang Autonomous region,Nanning 530021 |
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| Abstract: | Objective：To analyze the frequency of azoosper-mia factor （AZF） microdeletions on Y chromosome in male infertility patients and discuss the value of AZF microdeletions on Y chromosome detections in male disorders.Methods：Multiplex polymerase chain reaction and agarose gel electrophoresis tests were used to detect 3 sequence tagged sites （STS） of AZF microdeletions in 150 male infertility patients.Results：AZF microdeletions were detected in 22 patients （14.67%）.Among 3 STS,deletions of the AZFc region of the Y chromosome were the most common,next was AZFb region,and deletions of AZFa region were the least common.Conclusion：AZF microdeletions were highly relevant to spermatogenesis defect.It is necessary to detect AZF microdeletions on Y chromosome in male infertility patients. |
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| Keywords: | Y chromosome microdeletions Azoospermia factor Male infertility |
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