MTRR基因多态性与2型糖尿病合并大血管病变的相关研究

目的研究蛋氨酸合成酶还原酶(MTRR)基因多态性与2型糖尿病合并大血管病变的关系.方法采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)法对175例正常人群、240例2型糖尿病、176例2型糖尿病合并大血管病变(脑梗塞104例、合并冠心病61例、糖尿病足11例)、87例非糖尿病的大血管病变组人群MTRR的A66G进行基因分析.比较上述各组基因型和等位基因频率分布有无差异.结果 4组间等位基因频率分布差异无显著性 (P>0.05),但基因型频率分布差异有显著性 (P<0.05),其中2型糖尿病和2型糖尿病合并大血管病变组AA基因型频率低于正常人群和单纯大血管病变组(P<0.05),AG基因型频率高于正常人群和单纯大血管病变组(P<0.05).结论 MTRR A66G基因多态性可能与2型糖尿病及2型糖尿病合并大血管的发生相关,AG基因型可能是2型糖尿病、2型糖尿病合并大血管病变的危险因素,AA基因型可能是其保护因素.

3Polymorphism of methionine synthase reductase gene in type 2 diabetes mellitus with macrovascular disease

Objective To investigate association of methionine synthase reductase gene with type 2 diabetes mellitus with macrovascular disease. Methods The MTRR gene A66G polymorphism in 678 Chinese han people in Anhui region, including 175 normal subjects, 240 type 2 diabetes mellitus patients, 176 type 2 diabetes mellitus patients with macrovascular disease (104 with cerebral infarction, 61 with coronary heart disease, diabetic food 11) and 87 patients with macrovascular disease only (13 with coronary heart disease and 74 with cerebral infarction), were determined by PCR-RFLP technique. The genotypes frequency and allele frequency among these groups were compared. Results There were no significant differences of allelic frequencies of polymorphism A66G in MTRR gene among the four groups( P >0 05), but there were significant differences of genotype frequencies of polymorphism A66G in MTRR gene among them( P <0 05). The frequencies of genotype AA in type 2 diabetes mellitus and type 2 diabetes mellitus patients with macrovascular disease were lower than that of the normal subjects and the group of macrovascular disease( P <0 05). The frequencies of genotype AG in type 2 diabetes mellitus and type 2 diabetes mellitus patients with macrovascular disease were higher than that of the normal subjects and the group of macrovascular disease( P <0 05). Conclusion These results imply that MTRR A66G gene polymorphism is significantly associated with type 2 diabetes mellitus and type 2 diabetes mellitus with macrovascular disease. AG genotype may be a risk factor for type 2 diabetes mellitus and 2 type diabetes mellitus with macrovascular disease. AA genotype may be a protective factor for type 2 diabetes mellitus and type 2 diabetes mellitus with macrovascular disease.