液基薄层细胞学技术筛查宫颈癌临床应用研究

目的应用液基薄层细胞学技术筛查女性宫颈癌，并对部分患者检测人乳头状瘤病毒（HPV）-16、-18型。方法采用液基薄层细胞学技术对医院妇科门诊患者及妇科体检者651例进行宫颈癌筛查，并采用荧光定量PCR对28例细胞学阴性者和13例低级别病变以上患者检测HPV-16、-18型。结果651例样本检出低级别病变11例，高级别病变2例，检出率分别为1．69％和0．31％，总检出率为2．0％；28例细胞学阴性者检出HPV-16、-18型3例，感染率为7．14％；13例低级别以上患者检出HPV-16、-18型6例，感染率为46．15％，感染率差异具有统计学意义。结论液基薄层细胞学技术简便实用，适宜于女性宫颈癌筛查；联合HPV-16、-18型检测更有意义。

Clinical application of screening cervical cancer by thinprep cytology test

Objective To screen cervical cancer by thinprep cytology test (TCT) and detect human papilloma virus (HPV)-16, -18 in some patients.Methods TCT was applied to screening cervical cancer in 651 cases of gynecology outpatients and healthy examination people, while real-time fluorescence quantitive PCR was adopted to detect HPV-16 and -18 in 28 cases of subjects with normal cytology and 13 cases of patients with low grade or above lesion.Results In 651 cases of samples, 11 cases (1.69%) of low grade and 2 cases (0.31%) of high grade squamous intraepithelial lesion were detected with TCT, and the total detection rate was 2.0%. The detected cases (%) of HPV-16 and -18 were 3 (7.14%) in 28 cases of cytology negative women, and 6 (46.15%) in 13 patients with low grade or above lesion. The difference of infection rate was statistically significant.Conclusion Thinprep cytology test is simple and practical, suitable for screening of cervical cancer. It is more significant to detect HPV-16 and -18 simultaneously.