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| 人宫颈癌基因RNA干扰真核表达载体的构建及其稳定转染胰腺癌细胞株的建立 | |
| 引用本文： | 张盛,朱亚青,张国新,蒋佳凯. 人宫颈癌基因RNA干扰真核表达载体的构建及其稳定转染胰腺癌细胞株的建立[J]. 世界华人消化杂志, 2011, 0(14): 1463-1468 |
| 作者姓名： | 张盛 朱亚青 张国新 蒋佳凯 |
| 作者单位： | 常州市第三人民医院肝胆外科;南京医科大学第一附属医院消化科; |
| 基金项目： | 常州市社会发展科技计划基金资助项目,No.CS20102014~~ |
| 摘 要： | 目的:构建针对人宫颈癌基因2(HCCR2)有效靶点的RNA干扰真核表达载体,鉴定获得干扰质粒稳定转染的人胰腺癌PANC1细胞株.方法:设计并合成多个针对HCCR2基因的RNA干扰序列,并将其插入真核表达慢病毒载体pGCsi-H1/Hygro/NEGative,通过测序和与HCCR过表达载体共转染293T细胞后行West... |
| 关 键 词： | RNA干扰 人宫颈癌基因 PANC1细胞 稳定转染 |
Construction of eukaryotic expression vectors expressing siRNAs targeting the HCCR2 gene and their transfection into PANC1 cells |
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| Sheng Zhang,Ya-Qing Zhu,Jia-Kai Jiang. Construction of eukaryotic expression vectors expressing siRNAs targeting the HCCR2 gene and their transfection into PANC1 cells[J]. World Chinese Journal of Digestology, 2011, 0(14): 1463-1468 | |
| Authors: | Sheng Zhang Ya-Qing Zhu Jia-Kai Jiang |
| Affiliation: | Sheng Zhang,Ya-Qing Zhu,Jia-Kai Jiang,Department of Hepatobiliary Surgery,the Third People's Hospital of Changzhou,Changzhou 213000,Jiangsu Province,ChinaGuo-Xin Zhang,Department of Gastroenterology,the First Affiliated Hospital of Nanjing Medical University,Nanjing 210029,China |
| Abstract: | AIM:To construct eukaryotic expression vectors expressing small interfering RNAs(siRNAs) targeting the human cervical cancer oncogene 2(HCCR2) gene and transfect them into human pancreatic cancer cell line PANC1 to obtain a cell line stably transfected with the HCCR2 siRNA plasmid.METHODS:Multiple siRNAs targeting the HCCR2 gene were designed,chemically synthesized,and cloned into the eukaryotic expression vector pGCsi-H1/Hygro/NEGative.The resulting recombinant vectors were identif ied by direct sequencing... |
| Keywords: | RNA interference Human cervical cancer oncogene PANC1 cells Stable transfection |
| 本文献已被 CNKI 等数据库收录！ | |