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| WWOX基因转染对胆管癌细胞增殖、凋亡及侵袭的影响 | |
| 引用本文： | 朱凯,黄强,邵峰,任维华. WWOX基因转染对胆管癌细胞增殖、凋亡及侵袭的影响[J]. 世界华人消化杂志, 2011, 0(3): 251-256 |
| 作者姓名： | 朱凯 黄强 邵峰 任维华 |
| 作者单位： | 安徽医科大学附属省立医院普外科;安徽医科大学附属省立医院肝胆实验室; |
| 摘 要： | 目的:探讨WWOX基因转染胆管癌细胞株QBC939后对其增殖、凋亡与侵袭性的影响.方法:用脂质体转染法将WWOX重组真核表达质粒转染QBC939细胞,建立稳定表达WWOX基因的细胞株.将其分为以下3组:QBC939组,QBC939/con组和QBC939/WWOX组.荧光定量RT-PCR和Western blot法检测... |
| 关 键 词： | 胆管癌 基因表达 转染 增殖 凋亡 侵袭 含有WW结构域的氧化还原酶基因 |
WWOX gene transfection inhibits proliferation, accelerates apoptosis, and reduces invasion in human cholangiocarcinoma cell line QBC939 |
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| Kai Zhu,Qiang Huang,Feng Shao. WWOX gene transfection inhibits proliferation, accelerates apoptosis, and reduces invasion in human cholangiocarcinoma cell line QBC939[J]. World Chinese Journal of Digestology, 2011, 0(3): 251-256 | |
| Authors: | Kai Zhu Qiang Huang Feng Shao |
| Affiliation: | Kai Zhu,Qiang Huang,Feng Shao,Department of General Surgery,the Affiliated Provincial Hospital of Anhui Medical University,Hefei 230001,Anhui Province,China Wei-Hua Ren,Laboratory of Hepatobiliary Diseases,China |
| Abstract: | AIM: To investigate the effect of transfection with the WW domain-containing oxidoreductase (WWOX) gene on cell proliferation, apoptosis and invasion in human cholangiocarcinoma cell line QBC939. METHODS: A recombinant eukaryotic expression plasmid containing the WWOX gene was introduced into QBC939 cells by liposomemediated transfection. The mRNA and protein expression of WWOX in QBC939 cells stably transfected with the recombinant plasmid was detected by quantitative RT-PCR and Western blotting, respectiv... |
| Keywords: | Cholangiocarcinoma Gene expression Transfection Proliferation Apoptosis Invasion WW domain-containing oxidoreductase gene |
| 本文献已被 CNKI 等数据库收录！ | |