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| Src激酶在胃癌细胞来源的exosome促进肿瘤细胞增殖中的作用 | |
| 引用本文： | 曲晶磊,曲秀娟,刘云鹏,赵明芳,侯科佐,姜又红,杨向红. Src激酶在胃癌细胞来源的exosome促进肿瘤细胞增殖中的作用[J]. 世界华人消化杂志, 2011, 0(6): 557-561 |
| 作者姓名： | 曲晶磊 曲秀娟 刘云鹏 赵明芳 侯科佐 姜又红 杨向红 |
| 作者单位： | 中国医科大学附属第一医院肿瘤内科;中国医科大学附属第一医院肿瘤研究所;中国医科大学附属盛京医院病理科; |
| 基金项目： | 国家自然科学基金资助项目,No. 31000607~~ |
| 摘 要： | 目的:研究胃癌细胞来源的外泌体(exosome)对肿瘤细胞增殖的影响,初步探讨Src蛋白激酶在此过程中的作用.方法:采用离心超滤和蔗糖密度梯度超速离心的方法从胃癌SGC7901细胞的上清液中分离出胃癌细胞来源的exosome.透射电子显微镜下观察exosome形态.MTT法检测细胞增殖能力,Western blot检测... |
| 关 键 词： | 外泌体 Src 增殖 胃癌 |
Role of tyrosine kinase Src in gastric cancer exosomemediated promotion of tumor cell proliferation |
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| Jing-Lei Qu,Xiu-Juan Qu,Yun-Peng Liu,Ming-Fang Zhao,Ke-Zuo. Role of tyrosine kinase Src in gastric cancer exosomemediated promotion of tumor cell proliferation[J]. World Chinese Journal of Digestology, 2011, 0(6): 557-561 | |
| Authors: | Jing-Lei Qu Xiu-Juan Qu Yun-Peng Liu Ming-Fang Zhao Ke-Zuo |
| Affiliation: | Jing-Lei Qu,Xiu-Juan Qu,Yun-Peng Liu,Ming-Fang Zhao,Ke-Zuo,Department of Medical Oncology,the First Hospital of China Medical University,Shenyang 110001,Liaoning Province,China You-Hong Jiang,Institute of Cancer Research,China Xiang-Hong Yang,Department of Pathology,Shengjing Hospital,China Medical University,Shenyang 110004,China |
| Abstract: | AIM:To investigate the effect of gastric cancer exosomes on tumor cell proliferation and to evaluate the role of tyrosine kinase Src in this process.METHODS:Exosomes were isolated and purified from gastric cancer SGC7901 cells by serial centrifugation and sucrose gradient ultracentrifugation and observed by electron microscopy.Cell proliferation was measured by MTT assay.Protein expression was assayed by Western blot.RESULTS:Gastric cancer exosomes had a characteristic saucer-like shape that was limited by ... |
| Keywords: | Exosomes Src Proliferation Gastric cancer |
| 本文献已被 CNKI 等数据库收录！ | |