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| 小鼠不全性肠梗阻对Cajal间质细胞表型的影响 | |
| 引用本文： | 郭新,刘东海,黄旭,陆红丽,吴贻松,韩燕飞,许文燮. 小鼠不全性肠梗阻对Cajal间质细胞表型的影响[J]. 世界华人消化杂志, 2011, 0(18) |
| 作者姓名： | 郭新 刘东海 黄旭 陆红丽 吴贻松 韩燕飞 许文燮 |
| 作者单位： | 上海交通大学医学院生理学教研室;南通大学附属医院泌尿外科; |
| 基金项目： | 国家自然科学基金资助项目,No.10672103,No.31071011~~ |
| 摘 要： | 目的:探讨小鼠小肠慢性不全性肠梗阻导致自发性节律性收缩运动、电活动变化与Cajal间质细胞(ICC)表型转化的关系.方法:通过外科手术方法在小鼠回肠套入硅胶管,建立小鼠不全性肠梗阻模型.利用苏木精-伊红(H&E)染色,研究小肠平滑肌层形态学改变;利用常规生理和电生理技术观察环行平滑肌肌条自发性节律性收缩和慢波变化规律;利用全层平滑肌免疫组织化学方法,观察ICC表型标志酪氨酸激酶受体(c-kit)表达的变化.结果:小鼠不全性肠梗阻模型形成后14d,梗阻近端肠管显著扩张、小肠平滑肌层明显增生肥厚;小肠平滑肌自发性节律性收缩节律紊乱、幅度不规则、频率变慢;平滑肌静息膜电位去极化、慢波幅度变小、频率变慢;ICC表型标志c-kit表达显著减弱、甚至消失.结论:小鼠不全性肠梗阻导致机械和电活动紊乱与ICC表型转化导致的ICC数量减少和网络破坏有关.该模型为进一步研究ICC表型改变的细胞/分子机理提供了一个良好的实验模型. |
| 关 键 词： | Cajal间质细胞 酪氨酸激酶受体 慢波 不全性肠梗阻 |
Phenotype alterations of interstitial cells of Cajal in mice with partial intestinal obstruction |
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| Xin Guo,Dong-Hai Liu,Xu Huang,Hong-Li Lu,Yi-Song Wu,Yan-Fei Han,Wen-Xie Xu. Phenotype alterations of interstitial cells of Cajal in mice with partial intestinal obstruction[J]. World Chinese Journal of Digestology, 2011, 0(18) | |
| Authors: | Xin Guo Dong-Hai Liu Xu Huang Hong-Li Lu Yi-Song Wu Yan-Fei Han Wen-Xie Xu |
| Affiliation: | Xin Guo,Dong-Hai Liu,Xu Huang,Hong-Li Lu,Yi-Song Wu,Yan-Fei Han,Wen-Xie Xu,Department of Physiology,Medical College of Shanghai Jiao Tong University,Shanghai 200240,China Xin Guo,Department of Urology,the Affiliated Hospital of Nantong University,Nantong 226001,Jiangsu Province,China |
| Abstract: | AIM:To investigate changes in mechanic and electrical activities in intestinal smooth muscle and the phenotypes of interstitial cells of Cajal(ICC) in mice with partial intestinal obstruction.METHODS:A mouse model of partial mechanical ileal obstruction was induced by surgery.The histochemical technique was used to investigate morphological changes in the distended intestinal regions of model mice 14 d after surgical induction.Mechanic and electric activities were recorded in normal and distended intestinal... |
| Keywords: | Interstitial cells of Cajal c-kit Slow wave Chronic partial intestinal obstruction |
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