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| 艾灸对D-半乳糖致小鼠衰老模型海马突触蛋白的影响 | |
| 引用本文： | 王中林 焦瑞清. 艾灸对D-半乳糖致小鼠衰老模型海马突触蛋白的影响[J]. 针刺研究, 1997, 22(3): 162-164 |
| 作者姓名： | 王中林 焦瑞清 |
| 作者单位： | 南京中医药大学第二临床医学院!210029(王中林,徐恒泽,徐有功),南京大学(焦瑞清,吴馥梅) |
| 基金项目： | 江苏省教委自然科学基金 |
| 摘 要： | 本实验将空白对照组和D-半乳糖造模组及造模同时又艾灸其“百会”、“大推”穴的小鼠的海马粗制突触体的总蛋白进行SDS-聚丙烯酸胶凝胶平板电泳,老马斯亮兰染色,薄层扫描分析,观察艾灸对D-半乳糖所致小鼠海马的突触蛋白的影响,以探讨艾灸抗衰老作用可能的中枢机制。1材料与方法1．1材料健康3月龄的ICR雄性小鼠30只,体重309士Zg,由本校实验动物中心提供。丙烯酸胺（Acr）、甲叉双丙烯酸胺（Bis）、十二烷基磺酸钠（SDS）等均为FLUKA公司生产,低分子量标准蛋白为上海丽珠东风生物技术有限公司生产,D一半乳糖为上海试剂二厂生… |
| 关 键 词： | 艾灸 D－半乳糖 小鼠 衰老 海马突触蛋白 |
Effects of Stick Moxibustion on Hippocampal Synaptosomal Proteins of Mice-aging Model Induced by D-Galactose |
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| Abstract: | stick moxibustion on the "Baihui" and "Dazhui" of mice of aging model induced by D-galactose,and then hippocampus of mice in normal control group, modeling group and stick moxibustion groupwas gotton respectively, synaptosomal protein was extracted and analyzed by SDS -PAGE and Minorlayer scanning. The results showed that, in modeling group of D-galactose, changes of relative contentof eight types of protein in hippocampi were greater than 5 %, among them, 4 was up and 4 was down.Above 8 types of protein in stick moxibustion curing group, except for 36 KDa protein, other 7 types ofrelative content proteins changed compared with modeling group. Protein increased (or decreased) inmodeling group decreased (or increased) in curing group. It maybe suggest that stick moxibustion hasgood dual regulation to the disadvantageous effect of D- galactose on hippocampal synaptosomal proteins of mice |
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