Antiserum to polyacrylamide gel-purified simian virus 40 T antigen. |
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| Authors: | R B Carroll S M Goldfine J A Melero |
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| Affiliation: | Department of Pathology, New York University Medical Center, New York, New York 10016, USA |
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| Abstract: | Antisera used previously to assay SV40 T antigen have been produced in tumor-bearing hamsters. This report describes the production and characterization of antiserum produced by injection of rabbits with denatured large-T antigen (Ag) purified by immunoprecipitation and electrophoresis. The resulting antiserum reacts with native large-T Ag as determined by indirect immunofluorescence, complement fixation, and indirect immunoprecipitation assays and binds to species such as “small-T Ag” and previously described proteolytic fragments of large-T Ag. |
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| Keywords: | To whom requests for reprints should be addressed. |
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