miR-155、miR-146a在口腔扁平苔藓患者外周血和组织中的变化与相关性研究

目的 探讨miRNA（miR-155、miR-146a及miR-146b）在口腔扁平苔藓（oral lichen planus, OLP）患者外周血及病损组织中的表达及其与OLP临床特征的关系，为进一步探讨OLP的发病机制提供参考。方法 采用荧光定量PCR法，以U6基因为内参，分别检测OLP患者外周血及病损组织、健康对照外周血及组织中miR-155、miR-146a及miR-146b的相对含量。采用SPSS17.0软件包对数据进行统计学分析。结果 荧光定量PCR检测显示，与正常对照组相比，外周血 miR-155 表达水平在OLP 患者中显著增高， 病损组织中升高更加明显（组织中miR-155水平是外周血中的7.0倍）；而OLP患者外周血中miR-146a、miR-146b含量与对照组相比无显著差异（P=0.218，P=0.229），但OLP患者病损组织中miR-146a表达升高，显著高于对照组（P=0.003），且组织中miR-146a水平是外周血中的5倍。相关性分析结果显示，外周血中miR-155的表达水平与REU计分呈高度正相关（相关系数R=0.887，P<0.01）。结论 OLP患者外周血和组织中miR-155、miR-146a异常变化表明，miR-155、miR-146a可能与OLP的发生与演进有关，OLP可能是以局部炎症为主的慢性炎症性疾病。

Detection of miR-155, miR-146a in PBNCs and tissues from patients with oral lichen planus

PURPOSE To investigate the miRNA (including miR-155, miR-146a and miR-146b) expression in peripheral blood mononuclear cells (PBNCs) or lesions tissues in oral lichen planus (OLP) patients and healthy controls and the relationship between miRNA and OLP clinical features. METHODS The expression of miRNA（miR-155, miR-146a and miR-146b）was assessed by quantitative fluorescent PCR in peripheral blood cells or lesions tissues in OLP patients and healthy controls. U6 was used as reference gene. Statistical analysis was performed with SPSS17.0 software package. RESULTS Real-time PCR showed that the expression of miR-155 in OLP PBNCs was significantly higher than that in controls, miR-155 expression in OLP lesions tissues was 7.0 times higher than that of PBMCs. Correlation analysis showed that the level of miR-155 expression in PBMCs was positively correlated with REU score(correlation coefficient R=0.887, P<0.01). There was no significant difference in miR-146a and miR-146b expression in PBMCs between OLP and control group. On the contrary, the miR-146a expression in OLP lesions tissues was significantly upreguated than that in control group (P=0.003).CONCLUSIONS The results indicated that miR-155 and miR-146a might play important roles in the pathogenesis of OLP and OLP is likely a chronic inflammatory disease characterized by local inflammation.