牙周炎基础治疗前、后龈沟液中SFRP1总量的变化

目的 检测慢性牙周炎（chronic periodontitis， CP）患者牙周基础治疗前、后龈沟液中分泌型卷曲相关蛋白1（secreted frizzled-related protein-1，SFRP1）的总量，探讨SFRP1与CP的关系及其在CP活动中的意义。方法 选择中、重度CP患者22例为试验组，牙周健康者5例为对照组，分别在刮治前及刮治后1个月检查试验组的探诊出血指数(BI)、牙周探诊深度(PD)及临床附着丧失(CAL)，并于洁治后1周、刮治后l周及刮治后1个月收集试验组的龈沟液，采用酶联免疫吸附实验测定SFRP1总量，应用SPSS19.0软件包对数据进行统计学处理。结果 刮治前（洁治后1周）龈沟液中SFRP1总量在中、重度CP组、正常对照组分别为（40.80±4.85）pg、（33.42±2.24）pg，前者显著高于后者（P<0.05）。中、重度CP组刮治后l周、1个月龈沟液中SFRP1总量分别为（45.99+5.23）pg、（36.92±4.00）pg，刮治后1个月SFRP1总量均显著低于洁治和刮治后1周，而刮治后1周龈沟液中SFRP1总量显著高于洁治后1周及刮治后1个月（P<0.05）。结论 牙周炎患者牙周基础治疗1个月后BI、PD显著改善，且SFRP1在龈沟液中的总量随牙周炎症状况的改变而变化。

Effect of periodontal initial therapy on the amount of SFRP1 in gingival crevicular fluid in patients with periodontitis

PURPOSE To detect the change of secreted frizzled-related protein-1（SFRP1） in gingival crevicular fluid during periodontal initial treatment and explore the relationship between SFRP1 and the activity of chronic periodontitis. METHODS Twenty-two patients with moderate to severe periodontitis were selected, and 5 healthy volunteers were enrolled into the study as control group. The bleeding index(BI),periodontal probing depth(PD)and clinical attachment loss(CAL) were recorded at 1 week after supragingival scaling, one month after subgingival scaling. Gingival crevicular fluid (GCF) was collected at 1 week after supragingival scaling, one week after subgingival scaling, one month after subgingival scaling. The level of SFRP1 in GCF samples was detected by ELISA. SPSS19.0 software package was used for data processing. RESULTS The amount of SFRP1 in GCF of moderate to severe periodontitis group was (40.80±4.85) pg, and that of normal control was (33.42±2.24) pg at 1 week after supragingival scaling. The amount of SFRP1 in GCF was significantly higher in moderate to severe periodontitis compared to normal control group (P<0.05). In moderate to severe periodontitis group, the amount of SFRP1 in GCF significantly increased at l week after subgingival scaling (45.99±5.23) pg compared to 1 week after supragingival scaling and 1 month after subgingival scaling (36.92±4.00) pg (P<0.05); There was significant decrease in the amount of SFRP1 in GCF at 1 month after subgingival scaling,compared to 1 week after supragingival scaling and l week after subgingival scaling (P<0.05).CONCLUSIONS The BI, PD in the periodontitis groups were significantly improved at 1 month after initial therapy,and the amount of SFRP1 in GCF changed with different periodontal inflammation state．