骨髓间充质干细胞对实验性自身免疫性重症肌无力临床及免疫功能影响的研究

目的：探讨骨髓间充质干细胞（MSCs）对实验性自身免疫性重症肌无力（EAMG）临床及免疫功能的影响。方法：EAMG大鼠模型20只分为移植组（n=10）和模型对照组（n=10），并设正常对照组（n=10）。收集从Lewis鼠骨髓中分离、培养及鉴定后的MSCs，并调至密度为1×10^6／100μL LPBS。移植组经尾静脉注入MSCs悬液100μL，模型对照组及正常对照组同期给予100μL PBS。定期对移植组和模型对照组行体重测定、Lennon临床评分以及血浆中AChRa97—116-Ab含量测定，并采用RT—PCR和ELISA法分别检测3组大鼠外周血单核细胞（PBMCs）中T-bet、GATA-3以及血浆中IFN-γ和IL-4含量。结果：移植组Lennon临床评分、AChRa97—116-Ab含量以及T—bet、GATA-3、IL-4、IFN-γ水平均较模型对照组显著降低，与正常对照组比差异无统计学意义；模型对照组T—bet与IFN-γ以及GATA-3与IL-4均呈显著正相关。结论：MSCs移植可以有效减轻EAMG症状，其可能作用机制是抑制T—bet和GATA-3表达，从而下调AChR特异性T和B细胞异常的免疫应答。

Effects of Bone Marrow Mesenchymal Stem Cells on the Clinical Manifestation and the Immune Function of Experimental Autoimmune Myasthenia Gravis

Aim: To explore the effects of marrow mesenchymal stem cells(MSCs) on the clinical manifestation and the immune function of EAMG. Methods: 20 EAMG rats were determined and divided randomly into the transplantation group and the model control group,each group had 10 rats, and prepared 10 healthy rats as the normal control group. MSCs were obtained from Lewis rats and planted in plastic culture capsule,then cultured and expanded in vitro. MSCs were identified by flow cytometry. Transplantation group were inje...