组蛋白去乙酰化酶6在宫颈癌组织中的表达及其临床意义

目的:探讨组蛋白去乙酰化酶6(HDAC6)在宫颈癌组织中的表达及其临床价值。方法:采用免疫组织化学染色法检测63例宫颈癌组织、38例宫颈上皮内瘤变(CIN)组织和63例正常宫颈上皮组织中HDAC6蛋白的表达,并分析其与宫颈癌患者临床病理参数之间的关系。采用Westernblotting随机检测4例宫颈癌组织及自身相对应正常宫颈上皮组织中HDAC6蛋白的表达。结果:宫颈癌组织中HDAC6蛋白表达的阳性率显著高于CIN组织和正常宫颈上皮组织(P<0.05)。HDAC6蛋白表达与宫颈癌患者年龄和组织学分化无关(P>0.05),但与临床分期、浸润深度和淋巴结转移密切相关(P<0.01或P<0.05)。进一步的Westernblotting结果表明宫颈癌组织中HDAC6蛋白的表达均显著高于正常宫颈上皮组织(P<0.05)。结论:HDAC6有望成为评价宫颈癌恶性程度和预后的指标。

Expression of HDAC6 protein in cervical carcinoma tissues and its clinical significance

AIM:To investigate the protein expression of histone deacetylase 6(HDAC6) in cervical carcinoma tissues and its clinical value.METHODS: The method of immunohistochemistry was used to detect the protein expression of HDAC6 in 63 cases of cervical carcinoma tissues,38 cases of cervical intraepithelial neoplasia(CIN) tissues and 63 cases of normal cervical epithelial tissues.The relationships between the protein expression of HDAC6 and clinical pathological features were analyzed.The protein expression of HDAC6 in randomly selected 4 cases of cervical carcinoma tissues and paired normal cervical epithelial tissues was detected by Western blotting.RESULTS: Positive rates of HDAC6 protein expression in cervical carcinoma tissues were significantly higher than that in CIN tissues or normal cervical epithelial tissues,and there were obvious differences among the 3 groups(P<0.05).The protein expression of HDAC6 was not related to age and histological differentiation(P>0.05),but closely associated with clinical stages,invasive depth and lymph node metastasis(P<0.01 or P<0.05).Furthermore,the result of Western blotting demonstrated that the protein level of HDAC6 in cervical carcinoma tissues was markedly higher than that in normal cervical epithelial tissues.CONCLUSION: HDAC6 may be an important molecular marker for evaluating malignant degree and prognosis of cervical carcinoma.