完全弗氏佐剂诱导脾脏T淋巴细胞凋亡预防非肥胖性糖尿病鼠1型糖尿病

为探讨脾脏T淋巴细胞凋亡在完全弗氏佐剂 (CFA)预防非肥胖性糖尿病 (NOD)鼠糖尿病中的作用 ,4 2只NOD雌鼠随机分成CFA组 (2 1只 )和磷酸盐缓冲盐水 (PBS)组 (2 1只 ) ,3周龄时于鼠后脚板注射CFA 5 0 μl 只和PBS5 0 μl 只。按不同的观察时间分 6周、12周和 30周 3个观察组。采用TUNEL末端标记法和免疫组织化学方法观察脾脏T淋巴细胞凋亡情况。结果显示 :CFA诱导脾脏成熟的CD4 + T淋巴细胞凋亡 ,但不能诱导成熟的CD8+ T淋巴细胞凋亡 ;CFA能预防NOD鼠糖尿病的发生。提示CFA可通过诱导脾脏T淋巴细胞凋亡 ,以减少外周的CD4 + T淋巴细胞 ,达到预防NOD鼠 1型糖尿病

Complete Freund''s adjuvant-induced splenic T cell apoptosis in the prevention of diabetes in non-obese diabetic mice

Objective To study the apoptosis of T lymphocytes in the spleen and its role in complete Freund's adjuvant (CFA) preventing diabetes in non obese diabetic (NOD) mice. Methods Forty two NOD mice were divided into two groups randomly: CFA treated NOD female mice (6w, 12w, and 30w) and PBS treated NOD female mice (6w, 12w, and 30w ).The apoptosis of splenic T lymphocytes was observed with the TDT mediated fluorescein dUTP nick end labelling (TUNEL) technique and avidin biotin complex method. Results CFA induced the apoptosis of CD4 + T lymphocytes in the mouse spleen and could prevent the development of Type 1 diabetes (T1DM ) in NOD female mice.Conclusion CFA preventing Type 1 diabetes in NOD mice may be related to the apoptosis of T lymphocytes in the spleen, which downregulates mature CD4 + T lymphocytes in the periphery.