电纺丝技术制备生物活性复合纤维支架及其体外降解性

摘要 背景：聚乳酸/羟基磷灰石类复合材料支架常用的制备方法主要有冷压法、粒子沥滤法、热致相分离法等，但是在增强材料界面的结合、调节材料的降解速率、改善材料的强度等方面仍不能满足要求。 目的：制备左旋聚乳酸/羟基磷灰石复合纳米纤维支架。 方法：采用静电纺丝法制备聚乳酸/羟基磷灰石复合纳米纤维支架。以扫面电镜对纤维的结构形态进行分析，并观察其在PBS中浸泡不同时间的体外降解过程。 结果与结论：羟基磷灰石纳米粒子与聚乳酸/基体间存在化学键合，纳米粒子使纤维直径增大且表面粗糙程度增加，这种结构将有利于细胞在纤维膜上的伸展和和繁殖。羟基磷灰石的引入，抑制了聚乳酸降解过程中的自催化作用，减缓了聚乳酸的降解速度。说明电纺丝技术制备的聚乳酸/羟基磷灰石复合支架在组织工程支架材料方面具有潜在的应用前景。 关键词：电纺丝；羟基磷灰石；聚乳酸；降解性能；生物材料 doi:10.3969/j.issn.1673-8225.2011.08.010

Preparation and in vitro degradation of bioactive composite fiber scaffolds using electrospinning techniques

Abstract BACKGROUND: The common method of preparing poly(l-lactic acid) (PLLA)/hydroxyapatite (HA) composite scaffold includes cold-press method, granule filter method, heat partition method. But there are many respects that can not meet requirements in the interfacial combination, degradation rate, intensity of the materials. OBJECTIVE: To prepare a PLLA/HA composite nanofiber scaffold. METHODS: PLLA/HA composite nanofiber scaffold was prepared by electrospinning method. The structure and morphology were studied by scanning electron microscopy. The degradation in PBS was observed in vitro. RESULTS AND CONCLUSION: There was chemical bonding between HA nanoparticles and PLLA/base, the nanoparticles increased the fiber diameter and the surface roughness, this structure will be conducive to cell extension and reproduction in fiber membrane. HA inhibits the selfcatalys during the PLLA degradation, and slows down the degradation rates of PLLA. The PLLA/HA composite scaffold prepared by electrospinning technique may be potentially applied in tissue engineering scaffolds.