脂质体携前列腺素E_1对皮瓣缺血-再灌注损伤的保护作用

目的　探讨脂质体携载前列腺素E1 (PGE1 )对大鼠皮瓣缺血 再灌注损伤的保护机制。方法　制成大鼠下腹部岛状皮瓣缺血 再灌注损伤模型 ,将 32只大鼠随机分为 4组 ,用测量、称重以及组织化学、放射免疫和原子吸光度的方法进行观察。结果　脂质体携载PGE1 治疗明显减轻缺血 再灌组织及细胞线粒体的钙超载 (P <0 .0 1) ,抑制缺血 再灌注引起的血浆乳酸脱氢酶和组织蛋白酶D活性增加 (P <0 .0 1) ,降低血浆脂过氧化物丙二醛含量和血浆内皮素含量 (P <0 .0 5 )。脂质体携载PGE1 的治疗作用明显优于相同剂量的游离PGE1 的作用。结论　脂质体携载PGE1 对皮瓣缺血 再灌注损伤具有潜在临床治疗价值。

Protective effect of liposome carried prostaglandin E_1 on skin flap ischemia reperfusion injury in rats

Objective To explore the mechanism of protective effect of liposome carried prostaglandin E 1 (PGE 1) on rat skin flap ischemia / reperfusin(I/R). Methods Island flaps of abdomen of adult SD rats were established for ischmia reperfusion injury models. Weighting, immunocytochemistry and in situ hybridization were employed. Results It was found that treatment with liposome carried PGE 1 significantly alleviated I/R induced muscular edema, attenuated neutrophil accumulation in the I/R tissue ( P <0.01),inhibited calcium overload in the skelet muscle and cellular mitochondria ,lowered activities of plasma lactate dehydrogenase and cathpsin D ( P <0.01), and decreased plasma lipid peroxide formation and plasma endothelia content( P <0.05). The protective effects of liposome-carried PGE 1 were obviously better than that of free PGE 1 at the same dose. Conclusion Liposome carried PGE 1 may have a potential value for clinical therapy of I/R injury.