双氢青蒿素经皮给药体外透皮试验方法学研究

目的建立合适的双氢青蒿素（DHA）体外透皮试验方法。方法采用改良Franz扩散池法，以大鼠皮肤为透皮屏障，用HPLC法测定DHA累积透过量和体外透皮速率。结果DHA体外透皮速率以20％乙醇生理氯化钠溶液为接受液，比其他接受液高，达到32.158μg／h·cm^2；以20％乙醇生理氯化钠溶液为接受液测定油溶性软膏、卡波普水溶性软膏、泊洛沙姆水溶性软膏、O／W型乳膏、W／O型乳膏5种DHA软膏的透皮速率，分别为10．376、25．67、13．295、12．637、11．675μg,／h·cm^2。结论该方法准确、稳定，适用于DHA经皮给药的体外透皮试验研究。

Study on the Method ology of Transdermal Experiments in Vitro of Transdermal Drug Delivery of Dihydroartemisinin

Aim To establish a suitable method for transdermal experiments in vitro of dihydroartemisinin （DHA）. Methods By using an improved Franz diffusion cell and transdermal barrier of rat skin, the cumulative amount and transdermal permeation rate of DHA were determined by HPLC. Results DHA in-vitro permeation rate in the receptor liquid of 20% （V/V） ethanol physiological solution is higher than that of other receptor liquids, up to 32.158μg/h·cm^2. The permeation rate of oil-soluble ointment, O/W-cream, W/O-cream,two kinds of Carbopol and Poloxamer water-soluble DHA ointments was determined with 20% ethanol physiological solution. It was 10.376,12. 637,11.675,25.67,13.295μg/h·cm^2, respectively. Conclusion The method is accurate, stable and suitable for study on DHA transdermal experiments in vitro of transdermal drug delivery.