Ki-67、VEGF在胃肠间质瘤中表达及与MVD的相关性

目的:探讨Ki-67和VEGF在胃肠间质瘤中的表达及与临床病理因素的关系,VEGF、微血管密度(MVD)和细胞增殖之间的相关性。方法:采用免疫组化S-P法检测44例胃肠间质瘤组织中Ki-67、VEGF表达及计数MVD值和Ki-67PI。结果:VEGF、Ki-67在GIST组织中阳性表达分别为77.3%、63.6%,VEGF、Ki-67表达在不同大小的肿块之间有统计学差异(P<0.01);MVD值和Ki-67PI在VEGF阳性和阴性组的比较有统计学差异(P<0.01);VEGF、MVD和Ki-67PI之间呈显著正相关(相关系数分别为0.26、0.44和0.84,P<0.01)。结论:VEGF促进GIST组织中的新生血管形成,为肿瘤组织提供了丰富的血液和营养,并使细胞增殖活性增强,促进肿瘤细胞的增殖、肿瘤的生长、发展和转移;Ki-67PI为GIST的预后判断提供了比较客观的依据。

Expression of Ki-67 and VEGF in gastrointestinal stromal tumors and study on correlation among VEGF, microvessel density and carcinoma cell proliferation activity

Objective:To investigate the expression of vascular endothelial growth factor(VEGF)in gastrointestinal stromal tumor(GIST)and to expolore their ralations with clinicopathological features.To study the correlation among VEGF,microvessel density(MVD)and carcinoma cell proliferation activity.Methods:Expression of Ki-67 and VEGF were detected by immunohistochemical S-P technique in tissues of 44 cases of gastrointestinal stromal tumors.Results:Of 44 GIST,the positive rate of VEGF and Ki-67 expression were 77.3%(34/44)and 63.6%(28/44).The expression of VEGF and Ki-67 had significant difference in the size of tumors(P<0.01).The average MVD and Ki-67 proliferation index(Ki-67PI)were significantly higher in GIST with positive expression of VEGF than negative expression(P<0.01).There was a positive correlation between VEGF and MVD,VEGF and Ki-67PI(P<0.01).Conclusion:VEGF plays an important role in the promoting angiogenesis,development and metastasis of carcinoma cell in GIST.Ki-67PI may be used as an objective predictor for prognosis of GIST.