金属蛋白酶抑制基因RECK在肝细胞癌中的表达及生物学意义

目的探讨金属蛋白酶抑制基因RECK在肝细胞癌（hepatocellular carcinoma,HCC）中的表达及其临床意义。方法用实时逆转录聚合酶链反应（real-time RT-PCR）技术和免疫组化方法分别检测61例HCC、癌旁组织及18例正常肝组织中RECK mRNA和蛋白表达情况。结果 RECK mRNA在HCC中表达水平（0.0269±0.0026）低于癌旁肝组织（0.0378±0.0031,P〈0.05）。RECK蛋白在HCC组织中阳性表达率（59.0%）也显著低于癌旁肝组织（78.7%,P〈0.05）。RECK在HCC组织中的阳性检出率与肿瘤大小、是否复发、有无门脉癌栓、肿瘤有无完整包膜等临床因素显著相关（P〈0.05）,而与年龄、性别、有无肝硬化、乙肝表面抗原、AFP水平等无关。对61例患者的随访资料进行生存分析显示,RECK阳性组3年生存率83.9%,明显高于RECK阴性组52.1%（P〈0.05）。结论 RECK可能参与HCC的发生发展,有可能成为预测HCC浸润与转移的参考指标。

Expression and biological significance of matrix metalloproteinase inhibitor RECK gene in hepatocellular carcinoma

Objective To explore the expression and clinical significance of metalloproteinase inhibitor RECK gene in hepatocellular carcinoma（HCC）.Methods Real-time reverse transcription polymerase chain reaction（real-time RT-PCR） and immunohistochemical staining（IHC） were employed to determine the expression of RECK mRNA and protein in 61 human HCC tissues and their adjacent non-cancerous liver tissues.And the relationships between expression of RECK mRNA and protein in cancerous tissues and various clinical parameters were then analyzed.Results Both RECK mRNA expression levels by real-time RT-PCR and positive rates of RECK protein by immunohistochemistry in cancerous tissues（0.0269 0.0026 and 59.0%,respectively） were significantly lower than those in adjacent non-cancerous liver tissues（0.0378±0.0031 and 78.7%,respectively）（both P0.05 or 0.01）.Both expression levels of RECK mRNA and ptotein were negatively correlated with HCC size,recurrence,portal vein tumor thrombus and capsule state.Univariate analysis showed that RECK expression was significantly reversely correlated with the 3-year overall survival rate（P0.05）.Conclusion RECK may be involved in HCC development and progression and RECK may be useful to predicting metastasis and prognosis of HCC.