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| 大鼠压力负荷增加心肌肥厚模型中核仁素的表达 | |
| 引用本文： | 严思敏,吴双,孙丽,蒋碧梅,涂自智,肖献忠. 大鼠压力负荷增加心肌肥厚模型中核仁素的表达[J]. 中南大学学报(医学版), 2014, 39(2): 124-128. DOI: 10.11817/j.issn.1672-7347.2014.02.003 |
| 作者姓名： | 严思敏 吴双 孙丽 蒋碧梅 涂自智 肖献忠 |
| 作者单位： | 中南大学湘雅医学院 1. 2008级临床医学系；2. 病理生理学系，长沙 410078 |
| 基金项目： | 国家自然科学基金(项目编号：81170113),教育部新世纪优秀人才支持计划(NECT-12-0545).This,work,was,supported,by,the,grants,from,the,National,Natural,Science,Foundation,of,China(项目编号：81170113),Education,Ministry's,New,Century,Excellent,Talents,Supporting,Plan,P.R.China(项目编号：NECT-12-0545) |
| 摘 要： | 目的：探讨腹主动脉缩窄致压力负荷增加大鼠心肌肥厚模型中核仁素的表达情况。方法：采用体质量 180~220 g SD大鼠40只，随机分为假手术组和腹主动脉缩窄模型组，利用腹主动脉缩窄法制备压力负荷增加心肌肥 厚模型，分别于术后2周、4周观察心脏质量指数、左心室质量指数；采用RT-PCR检测心肌组织中 -MHC mRNA的 表达；采用Western印迹检测心肌、脑、肾组织中核仁素的表达情况。结果：腹主动脉缩窄模型组4周以后心脏质量 指数、左心室质量指数较假手术组显著增加(P<0.01)；4周以后心肌组织中 -MHC mRNA的表达较假手术组显著升高 (P<0.05)；2周以后心肌组织中核仁素蛋白的表达较假手术组显著升高(P<0.05)，而在脑、肾组织中无明显升高。结论： 核仁素蛋白在大鼠压力负荷增加心肌肥厚模型中的表达上调，表明核仁素可能参与了压力负荷增加心肌肥厚的发生 发展。 |
| 关 键 词： | 核仁素 心肌肥厚 大鼠 压力负荷增加 |
Expression of nucleolin in pressure overload-induced ardiac hypertrophy rats |
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| YAN Simin,WU Shuang,SUN Li,JIANG Bimei,TU Zizhi,XIAO Xianzhong. Expression of nucleolin in pressure overload-induced ardiac hypertrophy rats[J]. Journal of Central South University. Medical sciences, 2014, 39(2): 124-128. DOI: 10.11817/j.issn.1672-7347.2014.02.003 | |
| Authors: | YAN Simin WU Shuang SUN Li JIANG Bimei TU Zizhi XIAO Xianzhong |
| Affiliation: | 1. Clinical Medical Major, Grade 2008; 2. Department of Pathophysiology,Xiangya School of Medicine, Central South University, Changsha 410078, China |
| Abstract: | Objective: To detect the expression of nucleolin in cardiac hypertrophy rats induced by pressure overload. Methods: A total of 40 SD rats with body weight 180 g and 220 g were recruited and randomly divided into 2 groups: a transverse aortic constriction (TAC) group and a sham surgery group. Cardiac hypertrophy model was employed by transverse aortic constriction surgery. Then 2 weeks and 4 weeks after the experiment, the heart mass index (HMI), left ventricle mass index (LVMI) were measured. β-MHC mRNA in the heart tissue was detected with RT-PCR. Nucleolin in the heart, brain and kidney was respectively detected with Western blot. Results: Compared with the sham surgery group, HMI, LVMI in the TAC group increased significantly (P<0.01) 4 weeks after the surgery; the expression of β-MHC mRNA in the heart tissue increased (P<0.05) in the TAC group 4 weeks after the surgery; and the expression of nucleolin protein in the heart tissue of the TAC group was remarkably upregulated (P<0.05) 2 weeks after the surgery, with no change in the brain and kidney tissue between the 2 groups. Conclusion: Expression of nucleolin protein has been upregulated in response to pressure overload, which may suggest that nucleolin plays a role in cardiac hypertrophy induced by pressure overload. |
| Keywords: | nucleolin cardiac hypertrophy rat pressure overload |
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