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| 骨髓间充质干细胞移植联用褪黑素对大鼠脊髓损伤修复的影响 | |
| 引用本文： | 丁华,袁志诚,狄荣科,章洪喜,汪雷,张晔. 骨髓间充质干细胞移植联用褪黑素对大鼠脊髓损伤修复的影响[J]. 郧阳医学院学报, 2010, 29(1): 5-8,F0003 |
| 作者姓名： | 丁华 袁志诚 狄荣科 章洪喜 汪雷 张晔 |
| 作者单位： | 丁华,袁志诚,章洪喜,汪雷(江苏大学附属人民医院骨科,江苏 镇江,212002);狄荣科,张晔(江苏大学解剖学教研室,江苏 镇江,212002) |
| 摘 要： | 目的:观察骨髓间充质干细胞(BMSCs)移植联用褪黑素(MT)对大鼠脊髓损伤修复的影响,并对其作用机制进行探讨。方法:首先采用密度梯度离心法分离培养大鼠BMSCs,以Brdu标记细胞核。然后将48只SD大鼠用改良Allen法制备T10脊髓损伤模型,并随机平均分为MT+BMSCs组、BMSCs组、MT组、模型对照组,另取12只作为空白对照组。分别于造模后1d、3d、7d、14d、21d行神经功能BBB评分及斜板试验,免疫组化检测带标记的BMSCs迁移情况,显微镜下观察移植后脊髓形态结构变化。结果:术后神经功能BBB评分及斜板试验结果MT+BMSCs组优于BMSCs组(P<0.05),BMSCs组优于MT及模型对照组(P<0.05);MT+BMSCs组及BMSCs组损伤阶段脊髓内可见Brdu标记阳性BMSCs存活,且阳性数目前者多于后者,灰质分布较白质多,以注射部位向周围损伤组织迁移;同时显微镜下可观察到MT+BMSCs组脊髓损伤区无明显空腔,空泡小而少,并可见较多神经纤维及细胞。结论:BMSCs联合MT移植促进大鼠损伤脊髓结构和功能恢复的效果明显优于单纯细胞移植组,两者联用具有协同效应。 |
| 关 键 词： | 脊髓损伤 骨髓间充质干细胞 褪黑素 移植 |
Influence of Bone Mesenchymal Stem Cells Transplantation Combined Melatonin on Repair of Spinal Cord Injury in Rats |
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| DING Hua,YUAN Zhi-cheng,DI Rong-ke,ZHANG Hong-xi,WANG Lei,ZHANG Ye. Influence of Bone Mesenchymal Stem Cells Transplantation Combined Melatonin on Repair of Spinal Cord Injury in Rats[J]. Journal of Yunyang Medical College, 2010, 29(1): 5-8,F0003 | |
| Authors: | DING Hua YUAN Zhi-cheng DI Rong-ke ZHANG Hong-xi WANG Lei ZHANG Ye |
| Affiliation: | DING Hua1,YUAN Zhi-cheng1,DI Rong-ke2,ZHANG Hong-xi1,WANG Lei1,ZHANG Ye2 (1Department of Orthopedics,Renmin Hospital,2Department of Anatomy,Jiangsu University,Zhenjiang,Jiangsu 212002,China) |
| Abstract: | Objective To observe the influence of BMSCs transplantation combined with melatonin on repair of spinal cord injury in rats,and to explore its possible mechanism.Methods BMSCs were isolated and cultured with density gradient centrifugation from rats and the nucleus of BMSCs were labelled with Brud.The T10 spinal cord injury model was prepared with modified Allen's method in 48 rats,which were randomly divided into MT+BMSCs group,BMSCs group,MT group and model group,and another 12 healthy rats were selected ... |
| Keywords: | Spinal cord injury Bone mesenchymal stem cell Melatonin Transplantation |
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