核仁素在骨肉瘤组织中的表达及其意义

目的探讨骨肉瘤中核仁素蛋白的表达情况，明确其表达与骨肉瘤临床病理遗传的关系。方法骨肉瘤组织35例，骨软骨瘤组织15例，应用免疫组化技术，检测石蜡包埋组织中核仁素蛋白的表达，并分析核仁素蛋白的表达与骨肉瘤临床病理遗传的关系。结果（1）核仁素蛋白在骨肉瘤组织中的阴性、弱、中、强表达依次为8．6％、20％、34．3％、37．1％，与骨软骨瘤组织（分别为40％，46．7％，13．3％，0）相比，其表达水平显著上调（P〈0．01）。（2）核仁素蛋白在普通型骨肉瘤中阴性、弱、中、强表达依次为6．9％、13．8％、34．5％、44．8％，与骨旁骨肉瘤（分别为16．7％，50％，33．3％，0）相比，其表达水平上调（P〈0．05）。（3）核仁素蛋白在成骨型骨肉瘤中阴性、弱、中、强表达依次为5．9％、5．9％、29．4％、58．8％，成软骨型骨肉瘤中分别为0％、33．3％、33．3％、33．3％，成纤维型骨肉瘤中分别为16．7％、16．7％、50％、16．7％，不同亚型普通骨肉瘤组织中，核仁素蛋白的表达差异无统计学意义（P〉0．05）。结论核仁素蛋白的表达与骨组织病变的性质有关，其表达水平与骨肉瘤的病理遗传有关。

Study on the expression and significance of nucleolin in osteosarcoma

Objective To investigate the expression of nucleolin in osteosarcoma and its clinical significance.Methods The expression of nucleolin was evaluated by immunohistochemistry in paraffin-em bedded tissues from 35 cases of osteosarcoma and 15 cases of osteochondroma.Results Among the osteo sarcoma cases, 8.6％ were negative for nucleolin, 20％ weakly positive, 34.3％ moderate positive, 37.1％ strong positive.The positive rate of nucleolin in osteochondroma was 40％, 46.7％, 13.3％, 0％, respectively.The nucleolin expression showed significant difference in the osteosarcoma and osteochondroma (P ＜0.01).Among the conventional ostcosarcoma cases, 6.9％ were negative for nucleolin, 13.8％ weakly positive, 34.5％ moderate positive, 44.8％ strong positive.The positive rate of nucleolin in paros teal osteochondroma was 16.7％, 50％, 33.3％, 0％, respectively.The nucleolin expression showed re markable correlation with pathological and genetical classification (P ＜ 0.05).The positive rate of nucleo 1in in osteoblastic osteochondroma was 5.9％, 5.9％, 29.4％, 58.8％, while in chondroblastic osteochon droma 0％, 33.3％, 33.3％, 33.3％ and 16.7％, 16.7％, 50％, 16.7％ in fibroblastic osteochondro ma, respectively.The positive expressions of nucleolin were almost the same in each subtype of osteosarco ma (P ＞ 0.05).Conclusions The nucleolin expression had correlation with property of tumors in bone, and the positive expressions of nucleolin had correlation with pathological and genetic classification of osteo