DNA修复基因XPC和XPG单核苷酸多态性与肝细胞癌易感性的关系

目的 探讨广西地区DNA修复基因XPC(Ala499Va1、Lys939G1n)和XPG(His1104Asp)单核苷酸多态性与肝细胞癌(HCC)易感性的关系.方法 采用以医院为基础的病例对照研究.经组织病理学确诊的HCC患者500例,相同地区,年龄、性别和民族频数匹配的非肿瘤患者507例.采用TaqMan MGB实时荧光定量PCR检测XPC和XPG基因多态性,比较不同基因型与HCC患病风险的关系.分别用t检验和X2检验对计量资料和计数资料进行统计分析;采用非条件的Logistic同归计算比值比(OR)及其95%可信区间(CI).结果 与XPC基因Ala499Va1位点CC基因型相比,CT或者TT基因型与HCC患病风险无相关性(校正OR=1.34,95%CI:0.85～2.12;校正OR=1.30,95%CI:0.68～2.51);与Lys939G1n位点AA基因理相比,AC或者CC基因型与HCC患病风险无相关性(校正OR=1.20,95%CI:0.78～1.85;校正OR=1.81,95%CI:0.88～3.73).与XPG基因His1104Asp位点CC基因型相比,CG或者GG基因型与HCC患病风险尢相关性(校正OR=0.85,95%CI:0.56～1.27;校正OR=1.12,95%CI:0.67～1.87).分层分析结果显示,与携带XPC基因Lys939G1n位点AA基因型女性相比,携带AC+CC基因型的女性患HCC的风险增加2.17倍(95%CI:01～4.64).结论 XPC基因Ala499Va1和Lys939G1n位点以及XPG基因His1104Asp位点SNP的单独效应可能与HCC易感性无相关性,但是XPC基因Lys939G1n位点C等位基因可协同增加女性患HCC的风险.

Association of XPC and XPG polymorphisms with the risk of hepatocellular carcinoma

Objective To investigate whether the polymorphism of DNA repair genes XPC(Ala499Va1 and Lys939G1n)and XPG(His1104Asp)is associated with the susceptibility to hepatocellular carcinoma (HCC).Methods A hospital-based case-control study was conducted in 500 cases with HCC and 507 controls.Genotypes of XPC and XPG were determined by real-time polymerase chain reaction with the TaqMan MGB probe.Results Compared to the CC genotype,the CT genotype and the Tr genotype of XPCAla499Va1 were not associated with the susceptibility to HCC(adjusted OR=1.34,95%CI:0.85-2.12;adjusted OR=1.30,95%CI:0.68-2.51,respectively).Compared to the AA genotype,the AC genotype and the CC genotype of Lys939G1n were not associated with the susceptibility to HCC(adjusted OR=1.20,95%CI:0.78-1.85;adjusted OR=1.81,95%CI:0.88-3.73,respectively).Compared to the CC genotype,the CG genotype and the GG genotype of XPG His1104Asp were not associated with the susceptibility to HCC(adjusted OR=0.85.95%CI:0.56-1.27;adjusted OR=1.12,95%CI:0.67-1.87,respectively)However,the stratified analysis revealed that the females with the AC+CC genotype of XPC Lys939G1n had increasod risk of HCC compared to those with AA genotype(OR=2.17,95%CI:1.01-4.64).Conclusion Ourr esults suggest that XPC and XPG polymorphisms do not independently affect on the susceptibility to HCC,but the joint effect of C allele of XPC Lys939G1n and female may modify the risk of HCC.