| 广枣总黄酮对CVB3病毒引起细胞凋亡的影响 |
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| 引用本文： | 刘爽. 广枣总黄酮对CVB3病毒引起细胞凋亡的影响[J]. 中医药导报, 2011, 17(3): 87-89 |
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| 作者姓名： | 刘爽 |
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| 作者单位： | 内蒙古医学院第二附属医院,内蒙古,呼和浩特,010000 |
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| 摘 要： | 目的:建立病毒性心肌炎的体外模型,用新的化学方法提取到含量较高的广枣总黄酮,研究广枣总黄酮抗病毒的效果.方法:化学法提取广枣总黄酮,高效液相法测定其含量.培养HeLa细胞和乳鼠心肌细胞,在HeLa细胞和心肌细胞水平上对柯萨奇B3病毒进行毒力滴定,并测定广枣总黄酮的药物毒性,建立体外病毒性心肌炎模型,通过细胞病变抑制实验...
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| 关 键 词： | 广枣总黄酮 病毒性心肌炎 CVB3 细胞凋亡 |
The effect of TFC to cell apoptosis induced by CVB3 virus ABSTRACT |
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| Liu Shuang. The effect of TFC to cell apoptosis induced by CVB3 virus ABSTRACT[J]. GUIDING JOURNAL OF TCM, 2011, 17(3): 87-89 |
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| Authors: | Liu Shuang |
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| Affiliation: | Liu Shuang(second affiliated hospital of inner mongolia medical college,hohhot 010000) |
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| Abstract: | [Objectives]To establish model of viral myocarditis in vitro,To distill TFC(Total flavones of choerospondias axillaries fructus) with a new method,To test the anti-viral effect of TFC.Method:Using chemical means to distill TFC and testcontent with HPLC method.TCID50 of CVB3(coxsackievirus B3) was determined with Reed-Muench method,the anti-viral effect of TFC in vitro was validated through cytopathic effect experiment.Cardiomyocyte Apoptosis was performed by using Hoechst 33258、Flow cytometry.Result:The viral myocarditis model in vitro was successfully established,TFC can inhibit CPE(P<0.05)in CPE experiment.Virus-reproducing inhibiting experiment shows TFC can effectively inhibit coxsackievirus,Logarithm Value raise more than one unit,Apoptosis rate of cells was reduced.Conclusion:we established viral mycarditis model in vitro and proved TFC is an effective antiviral drug,TFC can reduce the rate of apoptosis induced by CVB3. |
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| Keywords: | CVB3 |
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