抗原抗体介导的细胞融合制备单克隆抗体

Monoclonal Antibody Production by Antigen-antibody Mediated Cell Fusion

Antigen (a new stomach tumor associated antigen) was incoporated into the membrane of myeloma cells utillizing a heterobifunctional regent SPDP. The myeloma cells coated by antigen were incubated with spleen cells from immunized mice, in this stage the myeloma cells selectively binded to antigen-reactive B-cells with the interposing antigen as a bridging ligand between the two cells. Then cell fusion was accomplished by using PEG as fusion agent. After 11 of these antigen-antibody mediated cell fusions,the result showed that 21. 2% of hybrids secreted specific antibodies. This is 5. 7 times higher than that we have observed in conventional cell fusion in our laboratory.