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| 直接肽段结合方法(DPRA)在中药注射剂过敏反应预测中的应用 | |
| 引用本文： | 张劲松,桑 晶,孙叶丹,匡 荣,洪利娅,许国峰. 直接肽段结合方法(DPRA)在中药注射剂过敏反应预测中的应用[J]. 中国药品标准, 2016, 17(4): 268-271 |
| 作者姓名： | 张劲松 桑 晶 孙叶丹 匡 荣 洪利娅 许国峰 |
| 作者单位： | 浙江省食品药品检验研究院,浙江省食品药品检验研究院,浙江省食品药品检验研究院,浙江省食品药品检验研究院,浙江省食品药品检验研究院,绍兴市现代医药公共服务中心 |
| 摘 要： | 目的:采用直接肽段结合方法（DPRA）考察两种中药注射剂的过敏反应，评估该方法在中药注射剂过敏反应检测中应用的可能性。方法:运用HPLC法，通过测定两种中药注射剂对半胱氨酸多肽和赖氨酸多肽的消耗，来推测其产生过敏反应的可能性。结果:两种中药注射剂对半胱氨酸多肽和赖氨酸多肽均有不同程度的消耗，预示有潜在的致敏性。结论:该方法可以作为中药注射剂过敏反应的预测方法。 |
| 关 键 词： | 直接肽段结合方法；中药注射剂；过敏反应；预测 |
| 收稿时间： | 2016-03-09 |
| 修稿时间： | 2016-07-19 |
The Application of Direct Peptide Reactivity Assay for Sensitization Prediction of Chinese Medicine Injection |
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| Affiliation: | Zhejiang Institute for Food and Drug Control,Zhejiang Institute for Food and Drug Control,Zhejiang Institute for Food and Drug Control,Zhejiang Institute for Food and Drug Control,Zhejiang Institute for Food and Drug Control,Shaoxing Modern Medcine Public Service Center |
| Abstract: | Objective: To evaluate the possibility of applying the method of Direct Peptide Reactivity Assay (DPRA) to predict allergic reaction in Chinese medicine injection,two kinds of Chinese medicine Injection were tested. Methods: Depletion of cysteine- and lysine-containing peptide were measured by high performance liquid chromatography (HPLC), which were used in assessment of sensitization for these two Chinese medicine Injections. Results: The results showed that there were some depletion of both cysteine- and lysine-containing peptide with these two Chinese medicine Injections, which indicated that these two Chinese medicine Injections had some potential of allergic reaction. Conclusion: DPRA can be used as a good method in assessment of sensitization for Chinese medicine Injection. |
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