| HGFK1基因真核表达载体的构建及鉴定研究 |
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| 引用本文： | 朱昱,顾玲玲,朱敏,张法标,方哲平.HGFK1基因真核表达载体的构建及鉴定研究[J].浙江医学,2016,38(16):1329-1332,1344. |
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| 作者姓名： | 朱昱 顾玲玲 朱敏 张法标 方哲平 |
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| 作者单位： | 浙江省台州医院肝胆外科 |
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| 基金项目： | 浙江省医药卫生科技计划项目；浙江省科技计划项目 |
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| 摘 要： | 目的构建携带人HGFK1基因的真核表达载体pcDNA3.1（-）-HGFK1。方法通过PCR方法从已经构建好的病毒穿梭载体中扩增出HGFK1基因，构建pcDNA3.1（-）-HGFK1真核表达载体，经PCR、酶切、测序、蛋白表达等方法进行鉴定。结果PCR、酶切、测序以及蛋白表达证实pcDNA3.1（-）-HGFK1载体序列正确。结论本研究成功构建了pcDNA3.1（-）-HGFK1真核表达载体，可为后续进行肝癌细胞的生物学研究提供帮助。
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| 关 键 词： | 基因 HGFK1 载体构建 |
Construction and identification of pcDNA3.1(-)-HGFK1 plasmid |
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| ZHU Yu,GU Lingling,ZhU Min,ZHANG Fanbiao and FANG Zheping.Construction and identification of pcDNA3.1(-)-HGFK1 plasmid[J].Zhejiang Medical Journal,2016,38(16):1329-1332,1344. |
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| Authors: | ZHU Yu GU Lingling ZhU Min ZHANG Fanbiao and FANG Zheping |
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| Institution: | Taizhou Hospital of Zhejiang Province |
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| Abstract: | Objective To construct human pcDNA3.1(-)-HGFK1 gene eukaryotic expression vector. Methods HGFK1 gene was amplified by PCR method from the constructed virus shuttle vector, and the pcDNA3.1 (-) -HGFK1 eukaryotic expression vector was constructed and identified by PCR, enzyme digestion, sequencing and protein expression. Results Enzyme digestion, PCR, sequencing and protein expression showed the successful construction of pcDNA3.1 (-)-HGFK1 eukaryotic expression vector. Conclusion We construct pcDNA3.1(-)-HGFK1 eukaryotic expression vector successfully, which may be used for further study of hepatocellular carcinoma gene therapy. |
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| Keywords: | Gene HGFK1 Vector Construction |
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