洞庭湖区东方田鼠天然抗日本血吸虫抗体水平的初步研究

目的：探讨洞庭湖区东方田鼠天然抗日本血吸虫尾蚴（ Ｃ Ａ）、童虫（ Ｊ Ａ）、成虫（ Ａ Ｗ Ａ）及虫卵抗原（ Ｓ Ｅ Ａ）的抗体检出率及其水平。方法：应用间接 Ｅ Ｌ Ｉ Ｓ Ａ 对未感染野生东方田鼠（ Ｗ Ｍ Ｆ）、室内繁殖东方田鼠（ Ｂ Ｍ Ｆ）和感染日本血吸虫尾蚴１５ ｄ 的室内繁殖东方田鼠（ Ｂ Ｍ Ｆｉ１５ ）的抗日本血吸虫 ４ 种不同阶段抗原的抗体进行了检测，并与昆明小鼠感染尾蚴１５ ｄ（ Ａ Ｍｉ１５ ）、４２ ｄ（ Ａ Ｍｉ４２）和未感染组（ Ａ Ｍ）作了对比。结果： Ｗ Ｍ Ｆ和 Ｂ Ｍ Ｆ的抗日本血吸虫抗体检出率以抗 Ｊ Ａ Ａｂ 最高（９４． ６％ 和 ９４． ４％ ）， 抗 Ａ Ｗ Ａ Ａｂ 其次（８５． ５％ 和 ８３． ３％ ）， 抗 Ｓ Ｅ Ａ Ａｂ （５８． １％ 和５９．４％ ），抗 Ｃ Ａ Ａｂ（１４．３％ 和１３．９％ ）最低。 Ｂ Ｍ Ｆｉ１５ 的４ 种抗体水平较未感染组显著升高， 抗 Ｊ Ａ Ａｂ 和抗 Ａ Ｗ Ａ Ａｂ 的检出率为 １００％ ，抗 Ｓ Ｅ Ａ Ａｂ 为８４．４％ ，抗 Ｃ Ａ Ａｂ 为６５．６％ ； Ｗ Ｍ Ｆ、 Ｂ Ｍ Ｆ和 Ｂ Ｍ Ｆｉ１５ 的４ 种抗体水平呈相同趋势（ Ｊ Ａ＞ Ａ Ｗ Ａ＞ Ｓ Ｅ Ａ＞ Ｃ Ａ）， Ｂ Ｍ Ｆｉ１５

PRELIMINARY STUDY ON THE LEVELS OF NATURAL ANTIBODIES AGAINST SCHISTOSOMA JAPONICUM IN MICROTUS FORTIS IN DONGTING LAKE AREA

AIM:To explore the detection rate and the levels of natural antibodies to cercarial antigen(CA),juvenile antigen(JA),adult worm antigen (AWA) and soluble egg antigen(SEA),respectively,derived from Schistosoma japoncum in Microtus fortis distributed in Dongting Lake area.METHODS:The antibodies in wild Microtus fortis (WMF),laboratory bred Microtus fortis (BMF)and laboratory bred Microtus fortis 15 days after S.japonicum infection (BMF i15 ) were detected by indirect ELISA and a comparison of the antibody levels between Microtus fortis including WMF,BMF,BMF i15 and albino mice without infection (AM), 15 days after infection(AM i15 ) and 42 days after infection (AM i42 ) was made.RESULTS:The positive rates of detection for anti JA Ab in WMF and BMF were 94.6%and 94.4%,respectively,followed by 85.5%and 83.3 % for anti AWA Ab,58.1% and 59.4% for anti SEA Ab,and 14.3% and 13.9% for anti CA Ab.The levels of antibodies to the above four antigens in BMF i15 increased significantly, the detection rates being 100% for anti JA Ab and anti AWA Ab, 84.4% for anti SEA Ab and 65.6% for anti CA Ab.The levels of antibodies in WME, BMF and BMF i15 had the similar trend(JA>AWA>SEA>CA),and those in BMF i15 to CA,JA,AWA and SEA increased by 1.9,2.2,2.1 and 1.5 folds,respectively,when compared with BMF.CONCLUSION:Natural antibodies to Schistosoma japonicum were found in wild and labortory bred Microtus fortis .The levels of antibodies in BMF i15 to four above antigens were apparently higher than those in AM i15 .