Plasma Cholecystokinin Responses after Ingestion of Liquid Meal and Intraduodenal Infusion of Fat, Amino Acids, or Hydrochloric Acid in Man: Analysis with Region Specific Radioimmunoassay |
| |
| Authors: | Seiichi Himeno Seiichiro Tarui Shuji Kanayama Toshio Kuroshima Vasuhisa Shinomura Chozo Hayashi Kayoko Tateishi Kenichi Imagawa Etsuro Hashimura Tushiyuki Hamaoka |
| |
| Institution: | Second Department of Internal Medicine. Central Laboratory for Clinical Investigation. Department of Oncogenesis. Institute for Cancer Research. Osaka University Medical School. Osaka;and Otsuka Pharmaceutical Co. Ltd. Tokushima, Japan |
| |
| Abstract: | A highly sensitive and precise radioimmunoassay system for plasma cholecystokinin (CCK) was developed with the anli-CCK-8 specific antiserum which raised against N-terminal amino acids residue of sulfated CCK-8 and reacted with CCK-8, CCK-33, and CK-39 but not with gastrin and its related peptides. Mean concentration of the fasting plasma CCK determined with this method using CCK-8 as standard was 12.9 ± 5.9 pg/ml in normal subjects (n = 26), and in patients with hepatic cirrhosis it was significantly higher (36.7 ± 16.9 pg/ml, n = 9, p < 0.01) than in normal subjects. In six young healthy volunteers, intraduodenal infusion of fat caused a significant increase ( p < 0.05) of plasma CCK from a basal level of 8.0 pg/ml to a peak of 43.0 ± 12.0 pg/ml at 20 min after starting of infusion. In the same subjects, a significant increase of plasma CCK was also observed by amino acids infusion, but no elevation of plasma CCK level was found during intraduodenal acidification. |
| |
| Keywords: | |
|
|
