神经生长因子对体外培养的人牙乳头间充质细胞ALP活性的影响

目的：观察神经生长因子（nerve growth factor NCGF)对人牙乳头间充质细胞ALP活性的影响。方法：酶组织化学法,结果：100U／ml NGF,在培养3d时即显著刺激人牙乳头间充质细胞ALP活性,培养5d和7d时,刺激作用进一步加强,且ALP活性在培养5、7d时明显高于3d者。NGF（100U／ml)在各培养时间点,对照组细胞ALP的分泌差异不显著。结论：NGF对人牙乳头间充质细胞ALP活性的刺激作用可能在成牙本质细胞的分化过程中具有重要意义。而促进细胞局部环境ALP的分泌可能与细胞间基南的生物矿化过程有关。

Effects of nerve growthfactor on ALP activity of human dental papilla mesenchyme cells cultured in vitro

AIM: To observe the effects of nerve growth factor(NGF) on ALPactivity of human dental papilla mesenchyme cells. METHODS: Enzymic histochemistry. RESULTS: NGF at 100U/ml increased ALP activity of the cells significantly at the culture time of 3d. When the cells were cultured up to 5 or 7 d, the stimulating effects were more evident and significanly higher than that cultured for 3d. NGF(100U/ml) was also shown to simulate the secretion of ALP from cultured cells at all observed times. Whereas, no significant difference was detected in the control group at different times of culture. CONCLUSION: The results suggested that the NGF might be involoved in the cascade of odontoblast differentiation, and the accumulation of ALP in local circumstances might participate in the biological mineralization process occurred in extracellular matrix.