Genetic association and gene expression studies suggest that genetic variants in the SYNE1 and TNF genes are related to menstrual migraine |
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Authors: | Astrid J Rodriguez-Acevedo Robert A Smith Bishakha Roy Heidi Sutherland Rod A Lea Alison Frith E Anne MacGregor Lyn R Griffiths |
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Institution: | 1.Genomics Research Centre, Institute of Health and Biomedical Innovation, Queensland University of Technology, Musk Ave, Kelvin Grove, Brisbane, QLD 4059, Australia;2.Clinithink Limited, Bridgend, UK;3.Centre for Neuroscience & Trauma, Blizard Institute of Cell and Molecular Science, London, UK |
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Abstract: | BackgroundMenstrual migraine (MM) encompasses pure menstrual migraine (PMM) and menstrually-related migraine (MRM). This study was aimed at investigating genetic variants that are potentially related to MM, specifically undertaking genotyping and mRNA expression analysis of the ESR1, PGR, SYNE1 and TNF genes in MM cases and non-migraine controls.MethodsA total of 37 variants distributed across 14 genes were genotyped in 437 DNA samples (282 cases and 155 controls). In addition levels of gene expression were determined in 74 cDNA samples (41 cases and 33 controls). Association and correlation analysis were performed using Plink and RStudio.ResultsSNPs rs3093664 and rs9371601 in TNF and SYNE1 genes respectively, were significantly associated with migraine in the MM population (p = 0.008; p = 0.009 respectively). Analysis of qPCR results found no significant difference in levels of gene expression between cases and controls. However, we found a significant correlation between the expression of ESR1 and SYNE1, ESR1 and PGR and TNF and SYNE1 in samples taken during the follicular phase of the menstrual cycle.ConclusionsOur results show that SNPs rs9371601 and rs3093664 in the SYNE1 and TNF genes respectively, are associated with MM. The present study also provides strong evidence to support the correlation of ESR1, PGR, SYNE1 and TNF gene expression in MM. |
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Keywords: | Migraine Menstrual migraine (MM) Menstrual related migraine (MRM) Pure menstrual migraine (PMM) ESR1 PGR TNF SYNE1 |
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