补肾疏肝方对神经性厌食应激模型大鼠下丘脑-垂体-卵巢轴功能的影响

目的：探讨补肾疏肝方对神经性厌食应激模型大鼠下丘脑-垂体-卵巢轴（hypothalamus-pituitary-ovarian axis,HPOA）功能的影响。方法：按照隔离、限食、束缚的方法建立神经性厌食大鼠应激模型,设立对照组、模型组、假操作组和补肾疏肝方组,每组10只,检测各组大鼠的体质量和动情周期的变化。在灌胃第35天、大鼠处于动情间期时收集血清和下丘脑、垂体组织标本,用放射免疫法检测血清雌二醇（estradiol,E2）、垂体卵泡刺激素（follicle stimulating hormone,FSH）、垂体黄体生成素（luteotrophic hormone,LH）和下丘脑β-内啡肽（β-endorphin,β-EP）,用酶联免疫法测定血清皮质酮（corticosterone,CORT）。结果：补肾疏肝方组大鼠体质量明显高于假操作组（P〈0.01）;动情周期紊乱率明显低于模型组和假操作组;血清E2和垂体LH水平明显高于模型组（P〈0.05）;下丘脑β-EP明显低于假操作组（P〈0.05）;血清CORT水平明显低于模型组（P〈0.05）,亦明显低于假操作组（P〈0.01）;垂体FSH水平高于模型组和假操作组,但差异无统计学意义。结论：补肾疏肝方可以提高神经性厌食应激模型大鼠的血清E2和垂体LH水平,可能是通过降低下丘脑β-EP和血清CORT水平,进而起到调控HPOA功能的作用。

Effects of Chinese herbal medicine Bushen Shugan Recipe in regulating the hypothalamus-pituitary-ovarian axis in a rat model of stress-induced anorexia

Objective：To investigate the effects of Bushen Shugan Recipe （BSSGR）,a compound traditional Chinese herbal medicine,in regulating the hypothalamus-pituitary-ovarian axis （HPOA） in a rat model of stress-induced anorexia.Methods：Anorexia was induced in rats by the methods of separation,diet restriction and constraint.Rats were divided into 4 groups randomly：control group,untreated group,sham-operated group and BSSGR group.After the experiments,body weights and oestrous cycles of the 4 groups were compared.The levels of serum estradiol （E2）,hypophysis luteotrophic hormone （LH）,hypophysis follicle stimulating hormone （FSH） and hypothalamus β-endorphin （β-EP） were detected by radioimmunoassay.The level of serum corticosterone （CORT） was detected by enzyme-linked immunosorbent assay.Results：Body weight of BSSGR group was significantly increased in comparison with sham-operated group（P0.01）; the oestrous cycle disordering rate was higher than those of the untreated group and sham-operated group; hypophysis LH and serum E2 were obviously increased in comparison with untreated group （P0.05）; hypothalamus β-EP was obviously decreased in comparison with sham-operated group （P0.05）; serum CORT was obviously decreased in comparison with untreated group （P0.05）,and significantly decreased in comparison with sham-operated group （P0.01）.Conclusion：BSSGR increased hypophysis LH and serum E2,and decreased serum CORT and hypothalamus β-EP in rats with stress-induced anorexia.