非那雄胺对大鼠前列腺微血管的影响

目的　评价非那雄胺对大鼠前列腺微血管的影响并探讨其机制。方法　非那雄胺 4 0 mg/ (kg· d)喂饲雄性 SD大鼠 7d(B组 ) ,14 d(C组 ) ,HE染色观察采用墨汁灌注染色的大鼠前列腺微血管密度 (MVD)的改变 ,免疫组织化学 SP法检测大鼠前列腺组织中血管内皮生长因子 (VEGF)及内皮型一氧化氮合酶 (e NOS)的蛋白表达。结果　 B组、C组的 MVD较对照组 (A组 )分别下降了 35 .2 % ,5 6 % ;B组、C组的 VEGF的蛋白表达较 A组分别减少 31.7%及 6 2 .6 % ;e NOS的蛋白表达较 A组分别减少 15 .7%及 5 2 .5 %。结论　非那雄胺可抑制大鼠前列腺组织中 VEGF、e NOS的蛋白表达进而抑制前列腺的微血管生成

Effects of Finasteride on Capillary in the Ventral Prostate of Rat

Objective To investigate the effects of finasteride on capillary in the ventral prostate of rat and study the mechanisms therein involved. Methods Male SD rats were given via gavage finasteride 40 mg/kg per day for 7 days (group B) and for 14 days (group C). The changes of capillary infused with Chinese ink were observed with the use of HE staining. Immunohistochemical (IHC) SP-method was adopted in measuring the protein expression of VEGF and eNOS in rat prostatic tissue. Results In comparison with the relevant values measured in the control group (group A), the microvessel density (MVD) values in group B and group C decreased by 35.2% and 56% respectively, the protein expression of VEGF in group B and group C decreased by 31.7% and 62.6% respectively, the protein expression of eNOS in group B and group C decreased by 15.7% and 52.5% respectively. Conclusion Finasteride can inhibit the protein expression of VEGF and eNOS and thereby can suppress angiogenesis of capillary in the ventral prostate of rat.